FOS (purity ≥95% and moisture ≤5%) was purchased from Shandong Baoling Bao Biological Co. Ltd., China. Tryptone, bile salt, yeast extract, L-cysteine, and heme were purchased from Sigma Company, USA. Phosphate-buffered saline (PBS), NaCl, KH2PO4, K2HPO4, MgSO4, CaCl2, metaphosphoric acid, and crotonic acid were purchased from Sangon Biotech (Shanghai) Co., Ltd., China.
Mgso4
Manufactured by Sangon
Sourced in China
MgSO4 is a chemical compound commonly known as magnesium sulfate. It is a white, crystalline solid that is highly soluble in water. MgSO4 is a widely used laboratory reagent and is commonly employed in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Kh2po4, by Sangon (3 mentions)
Cacl2, by Sangon (2 mentions)
K2hpo4, by Sangon (2 mentions)
Sodium chloride (nacl), by Sangon (2 mentions)
Yeast extract, by Merck Group (1 mentions)
Bile salt, by Merck Group (1 mentions)
L cysteine, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Sangon (1 mentions)
Tryptone, by Merck Group (1 mentions)
High temperature resistant α amylase, by Yuanye Bio-Technology (1 mentions)
Papain, by Yuanye Bio-Technology (1 mentions)
Sodium citrate, by Sangon (1 mentions)
Amyloglucosidase, by Yuanye Bio-Technology (1 mentions)
Beef extract, by Sangon (1 mentions)
Mnso4, by Sangon (1 mentions)
Tween 80, by Sangon (1 mentions)
Taq dna polymerase, by Takara Bio (1 mentions)
T4 dna ligase, by Takara Bio (1 mentions)
Na2s2o3, by Sangon (1 mentions)
Nh4 2so4, by Sangon (1 mentions)
5 protocols using mgso4
1
Reagent Procurement for Microbial Cultivation
2
Engineered E. coli Strains for Cinnamic Acid Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All strains and plasmids used and constructed in this study are listed in Table 1 . E. coli Trans-T1 was used as the general cloning host for plasmid construction and propagation. E. coli BL21(DE3) and its derivatives were used for enzyme expression and fermentation. The plasmids pRSFDuet-1, pETDuet-1 and pCDFDuet-1 were used as expression plasmids for recombinant plasmid construction.
All E. coli strains were grown with 90 mg/L trans-cinnamic acid in M9CA, YM9 or YNB medium for production. M9CA medium (1 L) consisted of 13.3 g M9CA broth (Sangon Biotech, Shanghai, China), 10 g glucose, 0.12 g MgSO4 and 0.5 mg VB1; YM9 medium (1 L) consisted of 11.3 g M9 salts (Sangon Biotech, Shanghai, China), 42 g MOPs (Sangon Biotech, Shanghai, China), 10 g yeast extract and 5% (v/v) glycerol [19 (link)]. For YNB medium, 100 mL of tenfold concentrated yeast nitrogen base without amino acids (Aladdin, Shanghai, China) and 10 g glucose was added to 900 mL base medium (6 g K2HPO4, 3 g KH2PO4, 10 g MOPS, pH 7.0).
All E. coli strains were grown with 90 mg/L trans-cinnamic acid in M9CA, YM9 or YNB medium for production. M9CA medium (1 L) consisted of 13.3 g M9CA broth (Sangon Biotech, Shanghai, China), 10 g glucose, 0.12 g MgSO4 and 0.5 mg VB1; YM9 medium (1 L) consisted of 11.3 g M9 salts (Sangon Biotech, Shanghai, China), 42 g MOPs (Sangon Biotech, Shanghai, China), 10 g yeast extract and 5% (v/v) glycerol [19 (link)]. For YNB medium, 100 mL of tenfold concentrated yeast nitrogen base without amino acids (Aladdin, Shanghai, China) and 10 g glucose was added to 900 mL base medium (6 g K2HPO4, 3 g KH2PO4, 10 g MOPS, pH 7.0).
3
Fermentation of Soybean Residue with Yeast and LAB
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fresh soybean residue was provided by Kangle Tofu Workshop (Changchun, China). Soybean residue should be dried for 24 h at 55 °C before reuse. Saccharomyces cerevisiae strain BNCC337309 was obtained from the BeNa Culture Collection (Beijing, China). The KC205 LAB strain was isolated and screened from homemade pickled cabbage juice by the food microbiology team of the Agro-product Process Institute of the Jilin Academy of Agricultural Sciences, and has the characteristics of strong acid production and acid resistance.
High temperature-resistant α -amylase (2 × 104 U/mL), papain (800 U/mg), and amyloglucosidase (1 × 105 U/mL), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Peptone, yeast extract fermentation, glucose, NaCl, beef extract, anhydrous sodium acetate, sodium citrate, K2HPO4, MgSO4, MnSO4 and Tween 80 were all purchased from Sangon Biotech Co., Ltd. (Shanghai, China). All chemicals were of analytical grade or better.
High temperature-resistant α -amylase (2 × 104 U/mL), papain (800 U/mg), and amyloglucosidase (1 × 105 U/mL), were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Peptone, yeast extract fermentation, glucose, NaCl, beef extract, anhydrous sodium acetate, sodium citrate, K2HPO4, MgSO4, MnSO4 and Tween 80 were all purchased from Sangon Biotech Co., Ltd. (Shanghai, China). All chemicals were of analytical grade or better.
4
Bacterial Genome Manipulation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Taq DNA polymerase, T4 DNA ligase and restriction enzymes (Xba I, Kpn I, Nhe I and Hind III) were purchased from Takara (Kyoto, Japan). Kits for genomic DNA exaction (E.Z.N.A.® Bacterial DNA Kit), plasmid extraction (E.Z.N.A.® Plasmid Mini Kit I) and DNA purification (E.Z.N.A.® Cycle Pure Kit) were purchased from Omega Bio-tek (Georgia, United States). Chemicals and reagents [Na2S2O3, K2S4O6, agar, (NH4)2SO4, KH2PO4, CaCl2, MgSO4, FeSO4, etc.] were purchased from Sangon (Shanghai, China) and Sinopharm (Shanghai, China). Primer synthesis and DNA sequencing were accomplished by GENEWIZ (Suzhou, China). All primers used in this study were listed in Supplementary Table S1 . The genomic sequences and all sequences of flagella and chemotaxis related genes were downloaded from the public National Center for Biotechnology Information (NCBI) website https://www.ncbi.nlm.nih.gov/ (Supplementary Table S2 ). BlastP and PsiBlast (Altschul et al., 1997 (link)) were used to further characterize candidate genes and their predicted protein products. Syntenic regions were displayed and analyzed with modification using Genomeviz (Ghai and Chakraborty, 2007 (link)).
5
Vibrio cholerae El Tor Biotype Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
V. cholerae El Tor biotype strain C6706 was used in this study. All experiments involving the live V. cholerae such as the bacteria culture and the bacteria inactivation were operated in the BSL-2 Laboratory.
Luria-Bertani (LB) medium was purchased from Oxoid (UK). Na2HPO4, KH2PO4, NaCl, NH4Cl, MgSO4, CalCl2, and glucose were purchased from Sangon Biotech (CHN). HPLC-grade acetonitrile (ACN), methanol (MeOH), and formic acid (FA) were purchased from Fisher Scientific (NJ, USA). Water was prepared by a Milli-Q system (Millipore, MA, USA).
Luria-Bertani (LB) medium was purchased from Oxoid (UK). Na2HPO4, KH2PO4, NaCl, NH4Cl, MgSO4, CalCl2, and glucose were purchased from Sangon Biotech (CHN). HPLC-grade acetonitrile (ACN), methanol (MeOH), and formic acid (FA) were purchased from Fisher Scientific (NJ, USA). Water was prepared by a Milli-Q system (Millipore, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!