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2 protocols using fitc conjugated anti igg3

1

B Cell Activation Pathway Assay

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FITC-conjugated anti-IgG3 and anti-IgA antibodies were purchased from BD-Pharmingen. APC-conjugated anti-B220, PE-conjugated anti-IgM, FITC-conjugated anti-IgG1, IL4, TGF-β, BLyS, and IL5 were from BioLegend. LPS was purchased from Sigma, anti-IgD-dextran from Fina Biosolutions, and anti-CD40 from eBiosciences. Anti-IgG antibody was purchased from Diagenode and anti-AID antibody from Abcam.
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2

Immunoglobulin Isotyping Flow Cytometry

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Allophycocyanin (APC)-conjugated anti-B220, fluorescein isothiocyanate (FITC)-conjugated anti-IgG1, Phycoerythrin (PE)-conjugated anti-IgG2b, PE-conjugated anti-IgG2a, and PE-conjugated anti-CD4 antibodies were purchased from BioLegend. FITC-conjugated anti-IgG3 and FITC-conjugated anti-IgA were from BD-Pharmingen. LPS was purchased from Sigma, anti-IgD-dextran from Fina Biosolutions, TGFβ, B-LyS, IFNγ and IL5 from R&D, and IL4 from eBiosciences.
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