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Mc120 hd microscope camera

Manufactured by Leica
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The MC120 HD Microscope Camera is a high-definition digital camera designed for integration with Leica microscopes. It captures detailed, high-resolution images for various scientific and research applications. The camera features a CMOS sensor and supports multiple video and image formats.

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Lab products found in correlation

Dm 1000 led microscope, by Leica (6 mentions) Las v 4, by Leica (5 mentions) Direct red 80, by Merck Group (3 mentions) Picrosirius red, by Adobe (2 mentions) Masson s trichrome mt, by Leica (2 mentions) Masson trichrome, by Leica (1 mentions) Histoclear solution, by National Diagnostics (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Potassium hexacyanoferrate, by Merck Group (1 mentions) Ab52625, by Abcam (1 mentions) M0851, by Agilent Technologies (1 mentions) Streptavidin hrp, by Biocare Medical (1 mentions) A5114, by Agilent Technologies (1 mentions) Aperio at2 slide scanner, by Leica (1 mentions)

Close spelling variants of this product

MC120 HD (48 citations) MC120 HD camera (29 citations) Camera microscope (2 citations)

9 protocols using mc120 hd microscope camera

1

Maize Leaf Anatomy Visualization

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Maize leaves (leaf 3) were dissected and cleared by incubating in 95% ethanol overnight. The samples were stained with iodine potassium iodide (IKI) solution (6) and excess IKI solution was washed off by rinsing in deionized water. Free-hand cross-sections were examined under bright-field under a Meiji Techno MT4310L dissecting microscope (Meiji Techno Co.) equipped with a Leica MC120 HD microscope camera.
Gao P., Wang P., Du B., Li P, & Kang B.H. (2022). Accelerated remodeling of the mesophyll-bundle sheath interface in the maize C4 cycle mutant leaves. Scientific Reports, 12, 5057.
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2

Histopathological Kidney Injury Assessment

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Kidneys were fixed in 10% neutral buffered formalin and embedded in paraffin. 5 ~m sections were cut for hematoxylin and eosin (H&E) and Masson’s trichrome (MTS) stainings (Leica). picrosirius red staining for collagen was performed using 0.1% picrosirius red (Direct Red 80, Sigma) and counterstained with Weigert’s hematoxylin. The extent of renal injury was estimated by morphometric assessment of tubular damage and interstitial fibrosis. To estimate the protection from tubular damage in UUO, NTN and folic acid mice, eight 200× visual fields were randomly selected for each slide and the number of healthy tubules was manually counted using the count tool of Adobe Photoshop. Tubules were defined as healthy when the dimension, structure, relative nucleus–cytoplasm disposition, integrity of the brush border and of the basal membrane resemble those of normal tubules from healthy kidneys. For the analysis of the interstitial fibrosis in UUO, NTN and folic acid mice, eight 200× visual fields were also randomly selected for each MTS and picrosirius red stained kidney section and interstitial fibrosis was manually evaluated by a grid intersection analysis using Adobe Photoshop. Representative images were acquired with Leica DM 1000 LED microscope and the MC120 HD Microscope Camera with Las V4.4 Software (Leica).
Lovisa S., LeBleu V.S., Tampe B., Sugimoto H., Vadnagara K., Carstens J.L., Wu C., Hagos Y., Burckhardt B.C., Pentcheva–Hoang T., Nischal H., Allison J.P., Zeisberg M, & Kalluri R. (2015). Epithelial to Mesenchymal Transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nature medicine, 21(9), 998-1009.
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3

Histopathological Assessment of Pancreatic Tumors

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Mouse tissues were fixed in 10% neutral buffered formalin, embedded in paraffin, and sectioned at 5 μm thickness. Sections were processed for hematoxylin and eosin (H&E) staining, Masson’s trichrome staining (MTS) using Gomori’s Trichome Stain Kit (38016SS2, Leica Biosystems), or picrosirius red (Direct Red 80, Sigma-Aldrich) according to manufacturer’s instructions. Histopathological assessments were conducted in a blinded fashion by scoring H&E-stained sections for relative percentages of the listed histopathological phenotypes. A weighted histology score was then applied to the percentages as follows: for tumors with less than 5% normal tissue two points, else zero points; greater than 30% PanIN or ADM tissue two points, else zero points; cancer area greater than 30% 4 points, else zero points; poor differentiated PDAC area greater than 30% 5 points, else zero points; and necrosis area greater than 5% 6 points, else zero points. The weighted scores were then summed for each animal to be interpreted as a higher value meaning worse histopathology. Images were obtained with a Leica DM 1000 LED microscope using a 20x objective and an MC120 HD Microscope Camera with Las V4.4 Software (Leica). Tumor scores for orthotopic tumors were evaluated based on H&E sections of the entire pancreas and attributed a score on a scale from 1 (minor involvement) to 4 (extensive involvement).
McAndrews K.M., Chen Y., Darpolor J.K., Zheng X., Yang S., Carstens J.L., Li B., Wang H., Miyake T., de Sampaio P.C., Kirtley M.L., Natale M., Wu C.C., Sugimoto H., LeBleu V.S, & Kalluri R. (2022). Identification of Functional Heterogeneity of Carcinoma-Associated Fibroblasts with Distinct IL-6 Mediated Therapy Resistance in Pancreatic Cancer. Cancer discovery, 12(6), 1580-1597.
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4

Histopathological Kidney Injury Assessment

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Kidneys were fixed in 10% neutral buffered formalin and embedded in paraffin. 5 ~m sections were cut for hematoxylin and eosin (H&E) and Masson’s trichrome (MTS) stainings (Leica). picrosirius red staining for collagen was performed using 0.1% picrosirius red (Direct Red 80, Sigma) and counterstained with Weigert’s hematoxylin. The extent of renal injury was estimated by morphometric assessment of tubular damage and interstitial fibrosis. To estimate the protection from tubular damage in UUO, NTN and folic acid mice, eight 200× visual fields were randomly selected for each slide and the number of healthy tubules was manually counted using the count tool of Adobe Photoshop. Tubules were defined as healthy when the dimension, structure, relative nucleus–cytoplasm disposition, integrity of the brush border and of the basal membrane resemble those of normal tubules from healthy kidneys. For the analysis of the interstitial fibrosis in UUO, NTN and folic acid mice, eight 200× visual fields were also randomly selected for each MTS and picrosirius red stained kidney section and interstitial fibrosis was manually evaluated by a grid intersection analysis using Adobe Photoshop. Representative images were acquired with Leica DM 1000 LED microscope and the MC120 HD Microscope Camera with Las V4.4 Software (Leica).
Lovisa S., LeBleu V.S., Tampe B., Sugimoto H., Vadnagara K., Carstens J.L., Wu C., Hagos Y., Burckhardt B.C., Pentcheva–Hoang T., Nischal H., Allison J.P., Zeisberg M, & Kalluri R. (2015). Epithelial to Mesenchymal Transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nature medicine, 21(9), 998-1009.
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5

Histological Analysis of Renal Injury

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Kidneys were fixed in 10% neutral buffered formalin and embedded in paraffin. 5 µm sections were cut for hematoxylin-eosin (H&E) and Masson trichrome staining (Leica). Masson trichrome staining for collagen was performed using 2% aniline blue and 0.7% acid fuchsin and counterstained with Weigert's ematoxylin (Beijing Zhongshan Jinqiao Biotechnology Co., LTD, China). The extent of renal injury was estimated using morphometric assessment of tubular damage and interstitial fibrosis. To evaluate the degree of damage to renal tubules in mice, eight 400× visual fields were randomly selected from each section, and the number of healthy renal tubules was manually counted using the Adobe Photoshop counting tool. Tubules were defined as healthy when the dimension, structure, relative nucleus-cytoplasm configuration, brush border, and basal membrane integrity were similar to those of healthy tubules. For the analysis of interstitial fibrosis of mice in each group, eight 400× fields were randomly selected from each Masson trichrome staining renal tissue section, and the degree of interstitial fibrosis was manually evaluated by Adobe Photoshop for grid cross analysis. A Leica DM 1000 LED microscope and MC120 HD microscope camera were used, and Las V4.4 software (Leica) was used to collect representative images.
Liu L., Ning X., Wei L., Zhou Y., Zhao L., Ma F., Bai M., Yang X., Wang D, & Sun S. (2022). Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis. Theranostics, 12(8), 3758-3775.
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6

Histological Analysis of Mouse Paws

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Mouse paws were fixed for 48 h in 4% paraformaldehyde. Fixed samples were processed sequentially as follows: decalcification for 21 days in an aqueous solution of hydrochloric acid (Sigma-Aldrich), dehydration in ethanol, clearing in Histoclear solution (National Diagnostics, Atlanta, GA, USA), infiltration, and embedding in paraffin. Serial sections (5 μm) were cut and stained with H&E. Histopathological features were examined under a Leica MC120 HD microscope camera (Wetzlar, Germany).
Oh J.S., Hwang S.U., Noh K.E., Lee J.H., Choi S.Y., Nam J.H., Song M.S., Jung N.C., Song J.Y., Seo H.G., Na Y, & Lim D.S. (2022). Synthetic TGF-β Signaling Agonist-Treated Dendritic Cells Induce Tolerogenicity and Antirheumatic Effects. Current Issues in Molecular Biology, 44(9), 3809-3821.
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7

Nuclear Fast Red Staining Procedure

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After the incubation of the cells with VSOP, the cells were washed two times with water prior to incubation with Nuclear Fast Red Stain solution for 30 min (Roth, Karlruhe, Germany). After washing with water, the cells were treated with a solution consisting of a 1:1 mixture of 2% HCl and 2% potassium hexacyanoferrate (Merck). The cells were mounted with coverslips and were photographed within 15 min using an inverse DMi1 microscope equipped with a MC 120 HD microscope camera (Leica, Wetzlar, Germany).
Freise C., Biskup K., Blanchard V., Schnorr J, & Taupitz M. (2024). Inorganic Phosphate-Induced Extracellular Vesicles from Vascular Smooth Muscle Cells Contain Elevated Levels of Hyaluronic Acid, Which Enhance Their Interaction with Very Small Superparamagnetic Iron Oxide Particles. International Journal of Molecular Sciences, 25(5), 2571.
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8

Histopathological Analysis of Metastases

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For paraffin‐fixed samples, mouse tissues were fixed in 10% neutral buffered formalin, embedded in paraffin, and sectioned at 5 μm thickness. In search for metastases, 20 sections for each tissue type (lung or liver) per mouse were randomly selected from the serial sectioning (with 100 μm interval) of the entire lung or liver lobe. Sections were processed for hematoxylin and eosin (H&E) staining. Microscopic metastases were examined in H&E‐stained tissue sections of the liver and lung. Images were captured with a Leica DM 1000 LED microscope and an MC120 HD Microscope Camera with Las V4.4 Software (Leica). Formalin‐fixed, paraffin‐embedded sections were processed for immunohistochemical staining as previously documented (Zheng et al, 2015). Sections were incubated with primary antibodies: αSMA (M0851, Dako, 1:100), CK19 (ab52625, Abcam, 1:200), or Fsp1 antibody (A5114, Dako, 1:100), then biotinylated secondary antibodies, and streptavidin HRP (Biocare Medical). For all immunolabeling experiments, sections were developed by DAB and counterstained with hematoxylin.
Chen Y., LeBleu V.S., Carstens J.L., Sugimoto H., Zheng X., Malasi S., Saur D, & Kalluri R. (2018). Dual reporter genetic mouse models of pancreatic cancer identify an epithelial‐to‐mesenchymal transition‐independent metastasis program. EMBO Molecular Medicine, 10(10), e9085.
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9

Histopathological Analysis of Mouse Kidney

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FIve-micron-thick sections of mouse kidney tissue were cut for hematoxylin and eosin (H&E). H&E-stained slides were imaged using Leica Aperio AT2 slide scanner or Leica DM 1000 LED microscope. Dysplasia and tumor were identified by histopathologic analysis and quantified as a percentage of total kidney area using Aperio Image Scope v12.3.2.8013 or ImageJ. The staining of Sirius Red and MTS were performed as described previously (14) . For each slide, three 200Â visual fields were randomly selected and representative images were taken by MC120 HD Microscope Camera (Leica). To estimate the tubular fibrotic damage of kidney, it was counted by grid intersection method manually via Adobe Photoshop.
Zhou X., Xiao F., Sugimoto H., Li B., McAndrews K.M, & Kalluri R. (2021). Acute Kidney Injury Instigates Malignant Renal Cell Carcinoma via CXCR2 in Mice with Inactivated Trp53 and Pten in Proximal Tubular Kidney Epithelial Cells. Cancer research, 81(10).
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