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Coolsnap hq2 camera

Manufactured by Prior Scientific
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The Coolsnap HQ2 camera is a high-performance, scientific-grade imaging device designed for a variety of microscopy and imaging applications. It features a high-resolution, cooled CCD sensor that captures detailed, low-noise images. The camera is capable of capturing images with a wide dynamic range and high sensitivity, making it suitable for a range of scientific imaging tasks.

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Lab products found in correlation

Alexafluor 647 conjugated runx2 antibody, by Novus Biologicals (2 mentions) Rhodamine cojugated phalloidin, by Biotium (2 mentions) Te2000 u microscope, by Nikon (1 mentions) Tcs sp8 upright, by Leica (1 mentions) Oil immersion objective, by Leica (1 mentions) Metamorph 7, by Universal Imaging (1 mentions)

3 protocols using coolsnap hq2 camera

1

Confocal and Spinning-Disc Microscopy Protocol

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Confocal microscopy was carried out with a TCS-SP8 upright confocal laser scanning microscope equipped with 63× oil immersion objective (NA = 1.4; Leica, Microsystems). Alexa 488 and GFP were excited at 488 nm and observed in a 505–540 nm window. Alexa 594 was excited at 594 nm and observed in a 600–630 nm window. Alexa 647 was excited at 633 nm and observed in a 650–700 nm window. For dual color acquisition, images were sequentially acquired in line scan mode (average line = 2). Overlays were performed with post acquisition Leica Confocal Software functions to obtain the presented snapshots. Golgi exit of granules was monitored by time-lapse fluorescence microscopy using a spinning-disc confocal microscope. This microscopy was carried out with a Yokogawa CSU-22 spinning-disc head on a Nikon TE-2000 U microscope equipped with a 100× NA 1.4 oil immersion objective and a Coolsnap HQ2 camera, a NanoScanZ piezo focusing stage (Prior Scientific) and a motorized scanning stage (Marzhauser). This microscope was steered with Metamorph 7.1 (Universal Imaging Corporation). The fast scan mode at 512 × 512 pixel resolution was used. Video sequences were acquired at 5 frames s−1 (100–200 ms exposures).
Delestre-Delacour C., Carmon O., Laguerre F., Estay-Ahumada C., Courel M., Elias S., Jeandel L., Rayo M.V., Peinado J.R., Sengmanivong L., Gasman S., Coudrier E., Anouar Y, & Montero-Hadjadje M. (2017). Myosin 1b and F-actin are involved in the control of secretory granule biogenesis. Scientific Reports, 7, 5172.
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2

Osteogenesis Induction in mMSC Microgels

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To induce osteogenesis, mMSCs encapsulated in microgels were cultured with complete DMEM supplemented with 10 mM β-glycerophosphate and 250 µM L-ascorbic acid, cycling every two days. mMSCs were fixed after three day of culture and stained, permeabilized with Triton X-100, blocked with 10% goat serum, and incubated with an AlexaFluor 647-conjugated Runx2 antibody overnight (Novus Biologicals, San Diego, CA). Imaging was performed with confocal microscopy (Upright Zeiss LSM 710). For assessing ALP activity, mMSCs were fixed six days after osteogenic induction and stained with elf-97, following the manufacturer's instructions. Staining was stopped through washing with excess of PBS after 90 seconds. Fixed cells were further stained with rhodamine-cojugated phalloidin (Biotium, Hayward, CA). Fluorescence images for immunohistochemistry, elf-97 staining, and alginate were acquired using an Olympus IX81 inverted microscope (BD Biosciences, San Jose, CA) and a Coolsnap HQ2 camera (Prior Scientific, Rockland, MA). The area-average fluorescence of cells stained with elf-97 and of alginate was quantified with ImageJ.
Mao A.S., Shin J.W., Utech S., Wang H., Uzun O., Li W., Cooper M., Hu Y., Zhang L., Weitz D.A, & Mooney D.J. (2016). Deterministic encapsulation of single cells in thin tunable microgels for niche modeling and therapeutic delivery. Nature materials, 16(2), 236-243.
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3

Osteogenesis Induction in mMSC Microgels

Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce osteogenesis, mMSCs encapsulated in microgels were cultured with complete DMEM supplemented with 10 mM β-glycerophosphate and 250 µM L-ascorbic acid, cycling every two days. mMSCs were fixed after three day of culture and stained, permeabilized with Triton X-100, blocked with 10% goat serum, and incubated with an AlexaFluor 647-conjugated Runx2 antibody overnight (Novus Biologicals, San Diego, CA). Imaging was performed with confocal microscopy (Upright Zeiss LSM 710). For assessing ALP activity, mMSCs were fixed six days after osteogenic induction and stained with elf-97, following the manufacturer's instructions. Staining was stopped through washing with excess of PBS after 90 seconds. Fixed cells were further stained with rhodamine-cojugated phalloidin (Biotium, Hayward, CA). Fluorescence images for immunohistochemistry, elf-97 staining, and alginate were acquired using an Olympus IX81 inverted microscope (BD Biosciences, San Jose, CA) and a Coolsnap HQ2 camera (Prior Scientific, Rockland, MA). The area-average fluorescence of cells stained with elf-97 and of alginate was quantified with ImageJ.
Mao A.S., Shin J.W., Utech S., Wang H., Uzun O., Li W., Cooper M., Hu Y., Zhang L., Weitz D.A, & Mooney D.J. (2016). Deterministic encapsulation of single cells in thin tunable microgels for niche modeling and therapeutic delivery. Nature materials, 16(2), 236-243.
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