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Recombinant nodal

Manufactured by R&D Systems
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Sourced in Germany

Recombinant NODAL is a protein produced using recombinant DNA technology. NODAL is a member of the transforming growth factor beta (TGF-β) superfamily of proteins and plays a critical role in embryonic development and cell differentiation.

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Lab products found in correlation

Ldn193189, by Merck Group (1 mentions) Noggin, by Abcam (1 mentions) Activin a, by R&D Systems (1 mentions) Tgf β1, by R&D Systems (1 mentions) Gemcitabine, by Eli Lilly (1 mentions)

Spelling variants of this product

Nodal (10 citations) Recombinant human nodal (2 citations)

2 protocols using recombinant nodal

1

Modulating TCam-2 Cell Differentiation

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TCam-2 cells were seeded 24h before treatment (1 x 105 cells /9,5 cm2). 500 ng /ml NOGGIN (diluted in 10 mM HAc) (Abcam, Cambridge, UK), 500 ng /ml LDN193189 (diluted in H2O) (Sigma-Aldrich, Taufkirchen, Germany) and 500 ng /ml recombinant NODAL (diluted in 4 mM HCl, 0.1% BSA) (R&D Systems, Wiesbaden, Germany) were added in 2 ml fresh culture medium every second day.
Nettersheim D., Jostes S., Sharma R., Schneider S., Hofmann A., Ferreira H.J., Hoffmann P., Kristiansen G., Esteller M.B, & Schorle H. (2015). BMP Inhibition in Seminomas Initiates Acquisition of Pluripotency via NODAL Signaling Resulting in Reprogramming to an Embryonal Carcinoma. PLoS Genetics, 11(7), e1005415.
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2

Gemcitabine and Growth Factors Preparation

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Gemcitabine (Gemzar, Lilly SA, Alcobendas, Spain) was resuspended to a working concentration of 1 µg/mL in PBS. Recombinant NODAL, ACTIVIN A and TGF-β1 were purchased from R&D Systems and resuspended according to the manufacturer's recommendations.
Cioffi M., Trabulo S.M., Sanchez-Ripoll Y., Miranda-Lorenzo I., Lonardo E., Dorado J., Reis Vieira C., Ramirez J.C., Hidalgo M., Aicher A., Hahn S., Sainz B J.r, & Heeschen C. (2015). The miR-17-92 cluster counteracts quiescence and chemoresistance in a distinct subpopulation of pancreatic cancer stem cells. Gut, 64(12), 1936-1948.
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