Plan apo λ 100 oil objective

SIM imaging was performed on a W1 spinning-disk microscope (CSU-W1; Nikon, Japan) combined with the Live-SR system (Roper Scientific) and equipped with a Plan-Apo λ 100× oil objective (1.45 NA; Nikon, Japan). Excitation and band-pass filters were as follows: DAPI was imaged with a 405-nm laser and emission of 445/45 nm, Alexa Fluor 488 was imaged using 488-nm excitation and emission of 525/30 nm, and Alexa Fluor 568 was imaged using 561-nm excitation and emission of 617/73 nm. Three-dimensional (3D) image stacks of whole cells were acquired with a scalable complementary metal-oxide semiconductor (sCMOS) camera (Prime 95B; Photometrics, USA), using an image pixel size of 78.96 nm by 78.96 nm (1,200 by 1,200 pixels) and an 0.2-μm z-stack step size. The raw images were processed to superresolution images by using the Live-SR algorithm reported by York et al. (21 (link)).