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Mrt67307

Manufactured by Selleck Chemicals
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MRT67307 is a laboratory equipment product. It functions as a tool for conducting scientific research and experiments in a laboratory setting.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Ribavirin, by Selleck Chemicals (2 mentions) Bx795, by Selleck Chemicals (2 mentions) Pegifnα 2a, by Roche (2 mentions) Recombinant murine tnf α, by Thermo Fisher Scientific (1 mentions) Il 1β, by Thermo Fisher Scientific (1 mentions) Jak inhibitor 1, by Merck Group (1 mentions) Lipopolysaccharides (lps), by List Biological Laboratories (1 mentions) P jnk, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Poly da dt lyovec, by InvivoGen (1 mentions) Fc blocker anti cd16 32, by Thermo Fisher Scientific (1 mentions) U0126, by Selleck Chemicals (1 mentions) Bx795, by MedChemExpress (1 mentions) Gsk2982772, by Selleck Chemicals (1 mentions) Poly 1 c, by Merck Group (1 mentions) Lipopolisaccaride lps, by Merck Group (1 mentions)

5 protocols using mrt67307

1

HEV Infectious Virus Stock Generation

Cited 1 time
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The infectious cDNA clones of HEV G1 (pSK-HEV-2 from the Sar-55 strain) (15 (link)) and G3 (p6 from the Kernow-C1 strain) were generous gifts from Dr. Suzanne U. Emerson (NIH, Bethesda, MD), whereas the infectious cDNA clone of G4 (pHEV-4TW from the TW6196E strain) has been described previously (37 (link)). The Huh7-S10-3 cell line was also a gift from Dr. Emerson. S10-3 cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) containing 10% FBS (Gibco), penicillin (250 IU/mL) and streptomycin (250 μg/mL) at 37°C in the presence of 5% CO2. MRT67307, BX795, and ribavirin were purchased from Selleck Chemicals, and peg-IFNα-2a was purchased from Shanghai Roche Pharmaceuticals.
The G3 Kernow-C1 HEV infectious virus stock used for gerbil inoculation was rescued from the p6 infectious cDNA clones in S10-3 cells as described previously (16 (link), 36 (link)). The viral RNA titer of the rescued infectious HEV stock was determined by real-time qRT-PCR (56 (link)), with 6.83 × 106 genome equivalents (GE) of viral RNA/100 μL medium, giving an infectivity of 1 fluorescent focus-forming unit (FFU)/5,618 GE, as determined previously (50 (link)).
Xu L.D., Zhang F., Chen C., Peng L., Luo W.T., Chen R., Xu P, & Huang Y.W. (2022). Revisiting the Mongolian Gerbil Model for Hepatitis E Virus by Reverse Genetics. Microbiology Spectrum, 10(2), e02193-21.
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2

Detailed Inflammatory Signaling Pathway Protocols

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Universal type I IFN was purchased from PBL biomedical laboratories. Cells were generally treated by IFN at 1000 U/ml or as specified. Recombinant murine TNFα and IL-1β were purchased from Peprotech. LPS was purchased from List Biological Laboratories, Inc. MEK/ERK inhibitor U0126 and TBK1/IKKε inhibitor MRT67307 were purchased from Selleckchem. Polyinosinic-polycytidylic acid sodium salt [poly (I:C)], Arsenic(III) Oxide and JAK inhibitor I were purchased from Sigma-Aldrich. Poly (dA:dT)/LyoVec was purchased from Invivogen. Antibodies used in western blot analyses: Tubulin (Sigma-Aldrich), β-actin (Sigma-Aldrich), p-p38 (Cell Signaling Technology), p-JNK (Cell Signaling Technology) and IkBα (Biolegend) were purchased from the respective manufacturers. Rabbit anti-mouse ISG15 polyclonal antibodies have been described previously (44 (link)). Fc blocker (anti CD16/32) was purchased from eBiosciences. A full list of antibodies is shown in Table S3.
Fan J.B., Miyauchi S., Xu H.Z., Liu D., Kim L.J., Burkart C., Cheng H., Arimoto K.I., Yan M., Zhou Y., Győrffy B., Knobeloch K.P., Rich J.N., Cang H., Fu X.D, & Zhang D.E. (2020). Type I Interferon Regulates a Coordinated Gene Network to Enhance Cytotoxic T Cell-Mediated Tumor Killing. Cancer discovery, 10(3), 382-393.
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3

Modulation of BMDM Activation Pathways

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BMDMs were stimulated with MRT67307 (Selleckchem, cat no. S7948), BX795 (Medchemexpress, cat no. HY-10514), 10 μM GSK2982772 (Selleckchem, cat no. S8484), or 20μM Necrostatin-s1 (Selleckchem, cat no. S8641) for the indicated times in the presence of 20 ng/ml mM-CSF.
Eren R.O., Kaya G.G., Schwarzer R, & Pasparakis M. (2024). IKKε and TBK1 prevent RIPK1 dependent and independent inflammation. Nature Communications, 15, 130.
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4

HEV Infectious Virus Stock Generation

Cited 1 time
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The infectious cDNA clones of HEV G1 (pSK-HEV-2 from the Sar-55 strain) (15 (link)) and G3 (p6 from the Kernow-C1 strain) were generous gifts from Dr. Suzanne U. Emerson (NIH, Bethesda, MD), whereas the infectious cDNA clone of G4 (pHEV-4TW from the TW6196E strain) has been described previously (37 (link)). The Huh7-S10-3 cell line was also a gift from Dr. Emerson. S10-3 cells were maintained in Dulbecco's Modified Eagle Medium (DMEM) containing 10% FBS (Gibco), penicillin (250 IU/mL) and streptomycin (250 μg/mL) at 37°C in the presence of 5% CO2. MRT67307, BX795, and ribavirin were purchased from Selleck Chemicals, and peg-IFNα-2a was purchased from Shanghai Roche Pharmaceuticals.
The G3 Kernow-C1 HEV infectious virus stock used for gerbil inoculation was rescued from the p6 infectious cDNA clones in S10-3 cells as described previously (16 (link), 36 (link)). The viral RNA titer of the rescued infectious HEV stock was determined by real-time qRT-PCR (56 (link)), with 6.83 × 106 genome equivalents (GE) of viral RNA/100 μL medium, giving an infectivity of 1 fluorescent focus-forming unit (FFU)/5,618 GE, as determined previously (50 (link)).
Xu L.D., Zhang F., Chen C., Peng L., Luo W.T., Chen R., Xu P, & Huang Y.W. (2022). Revisiting the Mongolian Gerbil Model for Hepatitis E Virus by Reverse Genetics. Microbiology Spectrum, 10(2), e02193-21.
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5

Antiretroviral and Innate Immune Activation

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3-Azido-3-deoxythymidine (zidovudine, AZT) was purchased from Sigma-Aldrich (Madrid, Spain). Raltegravir (RAL) was obtained from the NIH AIDS Research and Reference Reagent Program. MRT67307, a pharmacological inhibitor of IKKϵ and TBK1, was purchased from Selleckchem. When appropriate, differentiated macrophages were incubated with 100 ng/ml of lipopolisaccaride (LPS, Sigma-Aldrich) or 10 µg/ml of Poly I:C (Sigma-Aldrich), during4 hours at 37 °C.
Pujantell M., Riveira-Muñoz E., Badia R., Castellví M., Garcia-Vidal E., Sirera G., Puig T., Ramirez C., Clotet B., Esté J.A, & Ballana E. (2017). RNA editing by ADAR1 regulates innate and antiviral immune functions in primary macrophages. Scientific Reports, 7, 13339.
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