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2 protocols using celite fine 500

1

Tetronic 1107-Based Biopolymer Synthesis

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Tetronic® 1107 (T1107, molecular mass 15 k Da, HLB:18–23) was donated by BASF Corporation (Florham Park, NJ, USA). Acryloyl chloride, Celite fine 500, 4-dimethylaminopyridine (DMAP), succinic anhydride, N-hydroxysuccinimide (NHS), 4-methoxyphenol (99%), calcium hydride, tetrahydrofuran (THS) and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Toluene (HPLC grade), diethyl ether (anhydrous, BHT stabilized), and anhydrous sodium sulfate were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Dichloromethane (DCM) (HPLC grade), triethylamine (TEA), ditiothreitol (DTT), sodium bicarbonate, calcium hydride and d-chloroform (CDCl3) were purchased from Acros Organics (Pittsburg, PA, USA) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) from Tokyo Chemical Industry (Tokyo, Japan). Unless otherwise specified, all chemicals were of analytical grade and were used as received. Double-deionized ultra-filtered water was used throughout this study.
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2

Synthesis and Characterization of Tetronics-Based Polymer Conjugate

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Tetronics® T1107 (T1107, MW: 15 kDa, HLB: 18–23) was donated by BASF corporation (USA). Acryloyl chloride, Celite fine 500, 4-dimethylaminopyridine (DMAP), succinic anhydride, tetrahydrofuran (THF), N-hydroxysuccinimide (NHS), N, N-dicyclohexylcarbodiimide (DCC), and chloroform were purchased from Sigma-Aldrich (St. Louis, MO, USA). Toluene (HPLC grade), ethyl ether (anhydrous, BHT stabilized), and anhydrous sodium sulfate were purchased from Fisher Scientific (NJ, USA). Dichloromethane (DCM) (HPLC grade), triethylamine (TEA), ditiothreitol (DTT), sodium bicarbonate, calcium hydride and CDCl3 were obtained from Acros Organics (NJ, USA). DCM was dried with calcium hydride and stored over molecular sieves (Grade 514, Type 4A). All other chemicals were used as received.
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