Vectastain abc solution

Manufactured by Vector Laboratories

Sourced in United Kingdom, United States

Vectastain ABC solution is a ready-to-use reagent system designed for the sensitive detection of antigens in immunohistochemical and immunocytochemical procedures. It contains an avidin-biotin-peroxidase complex that amplifies the signal, providing enhanced visualization of the target antigen.

Automatically generated - may contain errors

Lab products found in correlation

Superfrost plus slides, by Avantor (2 mentions) Diaminobenzidine dab, by Vector Laboratories (1 mentions) Rabbit anti ki67, by Thermo Fisher Scientific (1 mentions) Normal donkey serum (nds), by Merck Group (1 mentions) Triton x, by Merck Group (1 mentions) Abe457, by Merck Group (1 mentions) Eclipse e600 light microscope, by Nikon (1 mentions) Dab substrate working solution, by Vector Laboratories (1 mentions) Dpx mounting medium, by Merck Group (1 mentions) Rat anti mouse cd68, by Bio-Rad (1 mentions) Histoclear, by National Diagnostics (1 mentions) Wheat germ agglutinin (wga), by Vector Laboratories (1 mentions) Neuronal nuclei (neun), by Vector Laboratories (1 mentions) Biotinylated horse anti mouse, by Vector Laboratories (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Anti neun, by Merck Group (1 mentions) Biotinylated goat anti rabbit, by Vector Laboratories (1 mentions) Biotinylated goat anti rabbit antibody, by Vector Laboratories (1 mentions) Cytoseal 60, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Vectastain ABC (102 citations) ABC solution (56 citations) Vectastain ABC-HRP solution (5 citations) Vectastain Elite ABC solution (4 citations) VECTASTAIN Elite ABC Kit solution (2 citations) VECTASTAIN ABC-alkaline phosphatase solution (2 citations)

7 protocols using vectastain abc solution

Neuroanatomical Tracing Technique

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brains were removed and postfixed in a 4% paraformaldehyde solution in 0.1M PBS and cryoprotected as above. In all cases, the BDA injections were made in combination with the fluorescent tracer Fluororuby in the contralateral hemisphere. For this tissue, a “1‐in‐4” series was mounted directly for fluorescence imaging and one for cresyl violet staining. Another series was collected free floating in 0.1M PBS before being washed three times, for 10 min each, in 0.1M PBST. Sections were incubated in the Vectastain ABC solution (Vector Labs, Peterborough, UK) for 2 h, then washed in PBST twice for 10 min each followed by a further three washes in 0.1M PBS. Sections were then reacted with diamino benzidine (DAB; Vector Labs, Peterborough, UK) and intensified with nickel after which they were mounted, dried and coverslipped, as described above.

Dillingham C.M., Erichsen J.T., O'Mara S.M., Aggleton J.P, & Vann S.D. (2015). Fornical and nonfornical projections from the rat hippocampal formation to the anterior thalamic nuclei. Hippocampus, 25(9), 977-992.

+ Open protocol

+ Expand

Peroxidase Immunohistochemistry for BrdU and Ki67

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peroxidase immunohistochemistry was performed essentially as described previously.^{43 (link)} Briefly, floating sections were incubated in hydrogen peroxide (1% in PBS) to destroy endogenous peroxidase activity; underwent antigen unmasking in 1 m HCL for 35 min at 37 °C (for BrdU only); were incubated in blocking buffer consisting of 0.2% Triton X (Sigma-Aldrich) and 5% v/v normal donkey serum (Merck-Millipore, Kilsyth, VIC, Australia) in 100 mm PBS for 1 h; were incubated in either sheep anti-BrdU (Exalpha Biologicals, Watertown, MA, USA) diluted 1/1000 or rabbit anti-Ki67 (Thermo Fisher Scientific Australia, Scoresby, VIC, Australia) diluted 1/200 in blocking buffer overnight at 20 °C; were incubated with biotinylated rabbit anti-sheep (Vector Laboratories, Burlingame, CA, USA) or biotinylated goat anti-rabbit Ki67 (Vector Laboratories) diluted at 1/500 for 2 h; incubated with Vectastain ABC solution (1:100, Vector Laboratories) for 1 h; developed with diaminobenzidine liquid chromogen kit (1:50, Dako Australia, North Sydney, NSW, Australia); were mounted on slides and then cover-slipped with DPX. Unless otherwise stated, each step occurred at room temperature and was followed by thorough washing with 100 mm PBS.

Rogers J., Vo U., Buret L., Pang T., Meiklejohn H., Zeleznikow-Johnston A., Churilov L., van den Buuse M., Hannan A, & Renoir T. (2016). Dissociating the therapeutic effects of environmental enrichment and exercise in a mouse model of anxiety with cognitive impairment. Translational Psychiatry, 6(4), e794-.

+ Open protocol

+ Expand

Immunohistochemical Quantification of cFos

Check if the same lab product or an alternative is used in the 5 most similar protocols

After completion of UBD, animals were injected with Euthasol and brains removed and processed as described above; for PBn cFos experiments, sham animals were catheterized but did not have bladders distended. Ten CeA or PBn sections identified using the Paxinos and Franklin Brain Atlas were incubated in 1% H₂O₂ in PBS for 5 minutes, rinsed with PBS, and then blocked in 10% normal goat serum (NGS) and 0.1% Triton X-100 in PBS for 1 hour. Sections were incubated with rabbit anti-cFos primary antibody (1:20,000, Millipore Cat #ABE457; in 3% NGS, 0.1% Triton X-100 in PBS) overnight at 4°C. After PBS wash, sections were incubated with goat anti-rabbit biotinylated immunoglobulin G (1:200, Vector; in 1% NGS in PBS) for 1 hour. Sections were rinsed with PBS, incubated in Vectastain ABC solution (Vector) for 1 hour, washed with PBS again, and then placed in DAB substrate working solution (Vector) for 60 seconds. After a final rinse in PBS, sections were mounted, coverslipped, and viewed using a Nikon Eclipse E600 light microscope. After delineating the CeA or PBn, cFos-positive cells were counted and averaged across sections for each animal using ImageJ software by an experimenter blinded to treatment and side of brain.

Sadler K.E., McQuaid N.A., Cox A.C., Behun M.N., Trouten A.M, & Kolber B.J. (2017). Divergent functions of the left and right central amygdala in visceral nociception. Pain, 158(4), 747-759.

+ Open protocol

+ Expand

CD68 Immunohistochemistry in Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Individual brain sections were mounted and dried on chrome gelatin-coated Superfrost-plus slides (VWR, Lutterworth, UK). To visualize CD68 immunoreactivity, sections were treated with 30% H₂O₂ in Tris-buffered saline (TBS) for 30 minutes. They were blocked with 15% rabbit serum in TBS-Tween 20 for 30 minutes. This was followed by the addition of primary antibody, rat anti-mouse CD68 (1:100; Bio-Rad, Watford, UK) in 10% serum, and TBS-Tween 20 and left overnight at 4°C. The following day the sections were treated with secondary biotinylated rabbit anti-mouse antibody (1:1000; Vector Laboratories, Burlingame, CA) and goat anti-rat (1:1000 dilution; Vector Laboratories) in 10% serum in TBS-Tween 20 for 2 hours. The sections were incubated for a further 2 hours with Vectastain ABC solution (Vector Laboratories). A total of 0.05% of 3,3′-diaminobenzidine was added and left for 2 minutes. Sections were transferred to ice-cold TBS. The slides were dehydrated in 100% ethanol and placed in Histoclear (National Diagnostics, Nottingham, UK) for 30 minutes before adding a coverslip with DPX mounting medium (Sigma-Aldrich, Dorset, UK).

Suff N., Karda R., Diaz J.A., Ng J., Baruteau J., Perocheau D., Tangney M., Taylor P.W., Peebles D., Buckley S.M, & Waddington S.N. (2018). Ascending Vaginal Infection Using Bioluminescent Bacteria Evokes Intrauterine Inflammation, Preterm Birth, and Neonatal Brain Injury in Pregnant Mice. The American Journal of Pathology, 188(10), 2164-2176.

+ Open protocol

+ Expand

Immunohistochemical Localization of NeuN and WGA

Check if the same lab product or an alternative is used in the 5 most similar protocols

NeuN and WGA (Vector Labs) were localised immunohistochemically (Horikawa & Powell, 1986). Initially, a 1‐in‐3 series of sections was washed (3 × 10 min) in 0.1 m PBS, followed by two further washes in 0.1 m PBST. Sections were then incubated for 48 h in a primary solution (anti‐WGA raised in goat; 1 : 2000 dilution, Vector Labs; anti‐NeuN; 1 : 5000, Chemicon, Chandlers Ford, UK) with 2% normal horse serum in 0.1 m PBST. Following incubation, sections were washed (3 × 10 min) in 0.1M PBST before being incubated for 2 h in a secondary antibody solution (for WGA, biotinylated rabbit antigoat at a 1 : 200 dilution; for NeuN, biotinylated horse antimouse at a 1 : 250 dilution; both from Vector Labs). After further washes in PBST (3 × 10 min), sections were incubated in the Vectastain ABC solution (Vector Labs) for 2 h, then washed in PBST twice for 10 min each followed by three further washes in 0.1M PBS. Sections were then reacted with diaminobenzidine (0.05% with 0.01%H₂O₂; DAB; Sigma–Aldrich). Once the desired level of staining was achieved, the reaction was stopped with washes in 0.1M PBS (3 × 10 min). Sections were then mounted, dehydrated and coverslipped, as above.

Christiansen K., Dillingham C.M., Wright N.F., Saunders R.C., Vann S.D, & Aggleton J.P. (2016). Complementary subicular pathways to the anterior thalamic nuclei and mammillary bodies in the rat and macaque monkey brain. The European Journal of Neuroscience, 43(8), 1044-1061.

+ Open protocol

+ Expand

Mapping Neuronal Activation via Immediate-Early Gene Immunoreactivity

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immediate-early gene immunoreactivity was used to regionally map neuronal activation as a function of IL stimulation. Briefly, sections were rinsed in KPBS, incubated in 0.03% hydrogen peroxide for 10 min, rinsed, and placed in blocking solution (KPBS, 0.1% BSA, and 0.2% TritonX-100) for 1 h at room temperature. Tissue was then incubated in rabbit polyclonal anti-Fos (sc-253) antibody (1:1,000; Santa Cruz) overnight at 4°C. This antibody labels multiple members of the Fra family, including Fra1, Fra2, c-Fos, and FosB (Ferrer et al. 1996 (link); Budzikowski et al. 1998 (link); Davern and Head 2007 (link)), and is a marker of recent neuronal activation (McClung et al. 2004 (link)). The following day, slices were rinsed and transferred into biotinylated goat anti-rabbit antibody (1:500; Vector Laboratories, Burlingame, CA) for 1 h and then rinsed again. Slices were incubated in Vectastain ABC Solution (1:1,000; Vector Laboratories) for 1 h and then rinsed and incubated in diaminobenzidine and hydrogen peroxide (0.02% diaminobenzidine/0.09% hydrogen peroxide in KPBS) for 10 min. Following incubation, slices were rinsed, slide mounted, dehydrated in graded ethanol, and cover slipped.

Wood M., Adil O., Wallace T., Fourman S., Wilson S.P., Herman J.P, & Myers B. (2018). Infralimbic prefrontal cortex structural and functional connectivity with the limbic forebrain: a combined viral genetic and optogenetic analysis. Brain structure & function, 224(1), 73-97.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Myelin Basic Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemical detection of myelin basic protein (MBP) was performed as described previously (Klocke et al. 2017 (link)). Briefly, whole brains (N=6-11/sex/exposure group; total N=33) were extracted at PNDs 11-15 following rapid decapitation and fixed in 4% paraformaldehyde (PFA) for 24 hrs and post-fixed in 30% sucrose. Post-fixed brains were sectioned sagittally at 40 μm in a 6-series on a freezing-sliding microtome into cryoprotectant and stored at −20º C until staining. Free-floating sections were washed to remove cryoprotectant and quench endogenous peroxidases, then blocked in 10% normal goat serum (NGS) followed by overnight incubation in a solution containing primary antibody against MBP (1:1000; Millipore, Billerica, MA) and 1% NGS. A single well of tissue incubated overnight in 1% NGS without primary antibody served as a negative control. Tissue was incubated with biotinylated secondary antibody and Vectastain ABC solution (Vector Labs, Burlingame, CA) the following day. Chromogenic antibody detection was then performed using metal-enhanced 3,3’-diaminobenzidinetetrahydrochloride (DAB; Sigma Aldrich, St. Louis, MO). Tissue sections were mounted onto Superfrost Plus slides (VWR, Radnor, PA) and coverslipped with Cytoseal 60 (Thermo Fisher Scientific, Waltham, MA).

Klocke C., Sherina V., Graham U.M., Gunderson J., Allen J.L., Sobolewski M., Blum J.L., Zelikoff J.T, & Cory-Slechta D.A. (2018). Enhanced Cerebellar Myelination with Concomitant Iron Elevation and Ultrastructural Irregularities following Prenatal Exposure to Ambient Particulate Matter in the Mouse. Inhalation toxicology, 30(9-10), 381-396.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!