The absorbance for determining the content of total flavonoids, total polyphenols, total polysaccharides, and total saponins was measured on a Shimadzu UV‐Vis spectrophotometer (UV‐1780, Shimadzu, Japan). Chemical composition analysis was performed on an Agilent 6545B Q‐TOF LC/MS using a Supelcosil ABZ+PLUS column (150 mm × 4.6 mm, 3 μm). Platelet aggregation induced by adenosine diphosphate (ADP) was measured using a semiautomatic platelet aggregometer (LBY‐NJ4, Beijing Precil Group, China). PT, APTT, and TT were tested by a C‐20004 semi‐auto coagulation analyzer (Beijing Precil Group, China). Coagulation test reagents were purchased from Shanghai TaiYang Biotechnology, China. DNS (3,5‐Dinitrosalicylic acid) and Folin–Ciocalteu's phenol reagent were purchased from Beijing Solarbio Science & Technology Co. Ltd. ADP was obtained from Ark Pharm, Inc. (Libertyville, IL, USA). Rutin, glucose, gallic acid, and saponin were purchased from Energy Chemical Technology Co., Ltd (Shanghai, China). Vanillin, perchloric acid, acetic acid, aluminum nitrate, sodium hydroxide, and other reagents were of analytical grade.
Folin ciocalteu s phenol reagent
Manufactured by Solarbio
Sourced in China
Folin-Ciocalteu's phenol reagent is a colorimetric reagent used for the determination of phenolic compounds in a sample. It is a mixture of phosphomolybdic and phosphotungstic acid complexes, which are reduced by phenolic compounds to produce a blue-colored complex that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Thermo Fisher Scientific (3 mentions)
Acetonitrile, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Gallic acid, by Solarbio (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
2 4 6 tri 2 pyridyl 1 3 5 triazine tptz, by Solarbio (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
2 4 6 tris 2 pyridyl s triazine, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid, by Merck Group (1 mentions)
Phloroglucinol, by Merck Group (1 mentions)
U 2910, by Hitachi (1 mentions)
Gallic acid, by Chengdu Must Bio-Technology (1 mentions)
Catechin, by Chengdu Must Bio-Technology (1 mentions)
Epicatechin, by Chengdu Must Bio-Technology (1 mentions)
Epicatechin gallate, by Chengdu Must Bio-Technology (1 mentions)
10 protocols using folin ciocalteu s phenol reagent
1
Comprehensive Phytochemical Analysis and Coagulation Assessment
2
Bioactive Compounds Characterization and Evaluation
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The standard of rutin was supplied by the National Institutes for Food and Drug Control (Beijing, China). Oroxin A, oroxin B, baicalein, chrysin, and oroxylin A were purchased from Must Biotechnology Co. Ltd (Chengdu, China). Gallic acid was purchased from Solarbio Science & Technology Co. Ltd (Beijing, China). α-glucosidase (EC 3.2.1.20, 32.4 U·mg−1), mushroom tyrosinase (EC 1.14.18.1, 1560 U·mg−1), L-DOPA, and 4-nitrophenyl-β-D-glucopyranoside (PNPG) were purchased from Baoman Biotechnology Co. Ltd (Shanghai, China). 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt (ABTS), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), and neocuproine was purchased from Aladdin Biochemical Technology Co. Ltd (Shanghai, China). 2,2-diphenyl-1-picrylhydrazyl (DPPH) was purchased from ApexBio Technology LLC (Houston, USA). 2,4,6-Tri(2-pyridyl)-1,3,5-triazine (TPTZ) and Folin & Ciocalteu's phenol reagent were purchased from Solarbio Science & Technology Co. Ltd (Beijing, China). Formic acid (analytical grade) was purchased from Thermo Fisher Technology Co. Ltd (Shanghai, China). HPLC-grad methanol, ethanol, and acetonitrile were obtained from Merck (Darmstadt, Germany). The ultrapure water used in this study was obtained by a Milli-Q water purification system (Millipore, Billerica, MA, USA). All other chemicals and reagents used were of analytical grade.
3
Comprehensive Analysis of Bioactive Compounds
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All references for qualitative and quantitative analysis were purchased from Chengdu DeSiTe Biotechnology Co., Ltd (Chengdu, China). 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-Azino-bis (3-ethylbenzothiazolie-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), neocuproine, and rutin were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). Tyrosinase, α-Glucosidase, p-Nitrophenyl-α-D-glucopyranoside (pNPG), L-Dopa were obtained from Shanghai Baomanbio Technology Co., Ltd (Shanghai, China). Gallic acid, Folin-Ciocalteu's phenol reagent and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Beijing Solarbio Technology Co., Ltd (Beijing, China). Ultrapure water was obtained from a Milli-Q system (Millipore, Billerica, MA, USA). Acetonitrile, methanol, ethanol and formic acid (both HPLC grade) were purchased from Merck (Darmstadt, Germany). All other chemicals and reagents were of analytical grade.
4
Extraction and Analysis of S. fusiforme Compounds
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The S. fusiforme sample was collected in 13 May 2014 in Dongtou (27°84′ N, 121°12′ E). The sample was washed with distilled water, then lyophilized. The dried seaweed was ground into powder and immediately stored at −20 °C until chemical analysis.
All solvents used were of HPLC grade. Phloroglucinol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Folin-Ciocalteu’s phenol reagent and a tea polyphenol product with a purity of 90% were obtained from Solarbio (Beijing, China). All other reagents used in this study were of analytical grade.
All solvents used were of HPLC grade. Phloroglucinol, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Folin-Ciocalteu’s phenol reagent and a tea polyphenol product with a purity of 90% were obtained from Solarbio (Beijing, China). All other reagents used in this study were of analytical grade.
5
Determination of Total Phenolic Content
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The determination of total phenolic content (TPC) was compiled by the Folin-Ciocalteu method of Lin et al. [28 (link)]. Apple samples (5 g) were ground to homogenates in methanol (5 mL) solution, and then extracted 3 times by ultrasound (15 min each time), and centrifuged at 10,000 r/min for 10 min, the supernatant was collected and diluted to 25 mL to prepare the sample solution. The reaction solution consisted of 400 μL sample solution, 600 μL distilled water, 1 mL Folin-Ciocalteu’s phenol reagent (Beijing Solarbio Technology Co., Ltd., Beijing, China), and 5 mL sodium carbonate (w/v, 20%) solution. The absorbance of the mixture was carried out after 30 min of reaction by an ultraviolet spectrophotometer (U-2910, Hitachi, Japan) at 725 nm, and the results were expressed as mg equivalents of gallic acid (GAE) per 100 g of fresh weight (FW) for each treatment.
6
Folin-Ciocalteu Phenol Content Assay
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The total phenol content was determined by Folin-Ciocalteu method, as modified by Cai [41 ]. The Folin-Ciocalteu’s phenol reagent was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). Different concentrations of gallic acid solution was mixed with 10% sodium carbonate solution in a 5 mL volume system. The absorbance of mixture was detected by spectrophotometer at 765 nm after incubation at 50 °C for 1 h. Total phenol content was calculated by the equation:
where C means the concentration calculated from standard curve, V means final volume of sample solution, N means dilution times, and m means the weight of sample.
where C means the concentration calculated from standard curve, V means final volume of sample solution, N means dilution times, and m means the weight of sample.
7
Quantitative Analysis of Polyphenols
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Formic acid, methanol, and acetonitrile (liquid chromatography–mass spectrometry (LC-MS) grade) were purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). Standards including gallic acid (GA, >98%), (+)-catechin (C, >98%), caffeine (CAF, >98%), (−)-epigallocatechin (EGC, >98%), (−)-epicatechin (EC, >98%), (−)-gallocatechin gallate (GCG, >98%), (−)-gallocatechin (GC, >98%), (−)-epicatechin gallate (ECG, >98%), and (−)-epigallocatechin gallate (EGCG, >98%) were purchased from Chengdu Must Bio-Technology (Chengdu, China). Folin–Ciocalteu’s phenol reagent was purchased from Solarbio Science & Technology Co., Ltd. (Beijing, China). DL-4-chlorophenylalanine was obtained from MedChemExpress (Shanghai, China). Ethylenediaminetetraacetic acid disodium (EDTA-2Na) was purchased from Sinop harm Chemical Reagent (Beijing, China). All other solvents and chemicals used in the tests were of analytical grade. The distilled water used in this study was obtained from Watsons Water Co. (Guangzhou, China).
8
Acid Protease Production by Aspergillus oryzae
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The A. oryzae strain 3.042 was purchased from China General Microbial Culture Collection Center (CGMCC No. 3.00951, Beijing, China) and used as the original strain for ARTP mutagenesis. Strain B-2 (CGMCC No. 8199) was a mutant strain with the supposedly highest acid protease activity by ARTP treatment of A. oryzae strain 3.042 in this study.
The bran and bean pulp were kindly supplied by Tianjin Tianli Mature Vinegar Co., Ltd. (Tianjin, China). Casein and Folin-Ciocalteu’s phenol reagent were purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). All other reagents were of analytical grade.
Potato dextrose agar (PDA) medium was used for the conventional culture and subculture of A. oryzae strain 3.042 and its mutants (Zhao et al., 2012 (link)). The screening medium was composed of 1% non-fat dry milk and 1.5% agar, which was sterilized at 115°C for 20 min before mixing, and the medium was adjusted to pH 3.0 using HCl under sterile conditions. The solid-state fermentation medium consisted of bean pulp, bran, and tap water at a ratio of 6:4:8 and was sterilized at 121°C for 30 min.
The bran and bean pulp were kindly supplied by Tianjin Tianli Mature Vinegar Co., Ltd. (Tianjin, China). Casein and Folin-Ciocalteu’s phenol reagent were purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). All other reagents were of analytical grade.
Potato dextrose agar (PDA) medium was used for the conventional culture and subculture of A. oryzae strain 3.042 and its mutants (Zhao et al., 2012 (link)). The screening medium was composed of 1% non-fat dry milk and 1.5% agar, which was sterilized at 115°C for 20 min before mixing, and the medium was adjusted to pH 3.0 using HCl under sterile conditions. The solid-state fermentation medium consisted of bean pulp, bran, and tap water at a ratio of 6:4:8 and was sterilized at 121°C for 30 min.
9
Phytochemical Analysis of Coffea arabica Leaves
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C4H6O4Zn•2H2O was purchased from Shanghai Aladdin Biochemical Technology Co. Ltd. (Shanghai, China). Folin-Ciocalteu's phenol reagent was purchased from Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). Gallic acid and NaBH4 were purchased from Sinopharm Chemical Reagent Co. Ltd. (Beijing, China). Phytochemical standards, including mangiferin, rutin, trigonelline, caffeine and chlorogenic acids (5-CQA: 5-caffeoylquinic acid; 3,4-diCQA: 3,4-dicaffeoylquinic acid; 3,5-diCQA: 3,5-dicaffeoylquinic acid; 4,5-diCQA: 4,5-dicaffeoylquinic acid) were purchased from Chengdu Must Bio-Technology Co. Ltd. (Chengdu, China). The fresh leaves of Coffea arabica were obtained from Hougu Coffee Plantation in Mangshi, Dehong County, Yunnan Province, China. All other reagents are analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd. (Zhenjiang, China).
10
Antioxidant Properties of Yinghong Tea
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Yinghong NO.9 samples yellowed for 0 hr (YT0h), 4 hr (YT4h), 8 hr (YT8h), 12 hr (YT12h), and 16 hr (YT16h) were provided by the Tea Research Institute, Guangdong Academy of Agricultural Sciences. ABTS and DPPH were obtained from Yuanye Biotechnology Co. Ltd. and TPTZ from MYM Biotechnology Co. Ltd. All catechins used for HPLC were obtained from Reference Research and Folin–Ciocalteu's phenol reagent from Solarbio Science & Technology Co. Ltd. All other chemical reagents were analytically pure and purchased from Damao Chemical Reagent Factory. Water was double‐distilled from the Laboratory Water Purification System (Ewell Bio‐Technology Co. Ltd.).
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