Dml b2 11888111 microscope

Manufactured by Leica

The DML B2/11888111 is a microscope from Leica, a leading manufacturer of high-quality optical equipment. The microscope is designed for use in laboratory settings, providing a reliable and precise tool for various applications. The core function of the DML B2/11888111 is to magnify and observe samples under controlled conditions.

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Lab products found in correlation

Dfc450, by Leica (3 mentions) Dfc450 camera, by Leica (3 mentions) Dm5500 b 11888817 12 microscope, by Leica (3 mentions) Alizarin red s, by Merck Group (2 mentions) L ascorbic acid, by Thermo Fisher Scientific (1 mentions) β glycerophosphate β gp, by Merck Group (1 mentions) Alizarin red s, by Molecular Devices (1 mentions) Elisa reader, by Molecular Devices (1 mentions) Alizarin red s, by Promega (1 mentions) Glomax fluorescence reader, by Promega (1 mentions) Dfc450 c camera, by Leica (1 mentions) Hi plan, by Leica (1 mentions) C plan, by Leica (1 mentions) Dfc450c, by Leica (1 mentions)

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DMLS microscope (43 citations) DML B2/11888111 (6 citations)

9 protocols using dml b2 11888111 microscope

Quantitative Histological and Immunohistochemical Kidney Assessment

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These measurements were carried out for histological and immunohistochemical quantitative assessment. Five non-overlapping fields per animal were randomly captured by a Lieca Microscope D.M.L. B2/11888111 equipped with a Leica camera DFC450. Kidney sections were randomly selected for morphometric measurements using image analyzer software (Image J analyzer version 1.43o8, National Institutes of Health, Bethesda, MD, USA). The following parameters were measured: the glomerular basement membrane (GBM) thickness (×200), the area percentage of collagen fibers stained by Masson trichrome stain (×200) and the area percentage of desmin and iNOs positive immune staining (×400).
Histological damage of the renal tubule was assessed as the percentage of the tubules that appeared dilated, atrophied, tubules with epithelial cell vacuolated cytoplasm, and with cast formation as 0 (normal), 1 (<10%), 2 (10 to 25%), 3 (26 to 50%), 4 (51 to 75%), and 5 (>75%). H&E-stained kidney segments were utilized to examine the renal tubular damage score. Five regions of the renal tubules were randomly chosen per kidney from each of the ten rats in each group for evaluation at a magnification of ×200, and the average score was calculated [30 ].

Negm W.A., El-Aasr M., Attia G., Alqahtani M.J., Yassien R.I., Abo Kamer A, & Elekhnawy E. (2022). Promising Antifungal Activity of Encephalartos laurentianus de Wild against Candida albicans Clinical Isolates: In Vitro and In Vivo Effects on Renal Cortex of Adult Albino Rats. Journal of Fungi, 8(5), 426.

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Quantifying Renal Morphometry and Immunohistochemistry

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For histological and immunohistochemical quantitative assessment, the sections from at least five animals/ experimental group were examined. Five non-overlapping fields per section were randomly captured by a Lieca Microscope DML B2/11888111 equipped with a Leica camera DFC450. Kidney sections were randomly selected for morphometric measurements by using image analyzer software (Image J analyzer version 1.43o8, National Institutes of Health, USA).
The following parameters were measured 1. The diameter of the renal corpuscle was measured X400
2. The area percentage of collagen fibers stained by Masson trichrome stain X200
3. The area percentage of (Desmin, VEGF & Keratin 18) positive immune-staining X400

Ragab R., El-Habiby M., El-Mehi A.E., & Faried M.A. (2020). The protective effect of anise, insulin, and their combination in the nephropathy of diabetic rat model. The Egyptian Journal of Histology, 0(0), 0-0.

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Quantitative Histological and Immunohistochemical Analysis

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For histological and immunohistochemical quantitative assessment, 5 non-overlapping fields (400×) per section were randomly captured by a Leica Microscope DML B2/11888111 equipped with a Leica camera DFC450. The number of immunopositive cells in the fields taken from at least three sections/animal was counted using ImageJ software (Maryland, USA) and averaged per field for each animal. The numbers calculated for at least 5 animals/experimental group were considered for comparison and statistical analyses.

Salman N.A.A.F.A., El-Safty F.E.A., El-Habeby M.M., El-Kholy W.B, & El-Akabawy G.F.A. (2019). Vitamin C attenuates the toxic effect of nutmeg on primary visual occipital cortex in rats. Folia morphologica, 78(1).

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Alizarin Red-S Staining for Osteoblast Differentiation

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The cells were seeded in 6-well plates at density of 0.8×10⁵ cells/well and stabilized for 24 h. To induce osteoblast differentiation, 50 µg/ml L-ascorbic acid (AA; Thermo Fisher Scientific, Inc.) and 10 mM β-glycerophosphate (β-GP; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) were added into osteogenic culture medium for 10 days. The culture medium was changed every 3–4 days. Then, the cells were fixed in 10% formalin for 10 min and stained with the 40 mM Alizarin Red-S (pH 4.2; Sigma-Aldrich; Merck KGaA) for 15 min, all at RT. The plates were observed under the Leica Microscope DML B2/11888111 equipped with Leica camera DFC450 at ×100 magnification. For quantification of Alizarin red S, 500 µl citrate solution containing 20% methanol and 10% acetic acid was added for 20 min at RT, and the absorbance of supernatants was measured at 570 nm using an ELISA reader (Molecular Devices, LLC., Downingtown, PA, USA).

Lee J.E., Lee H., Kim M.H, & Yang W.M. (2018). Osteogenic effects of Phlomis umbrosa via up-regulation of Runx2 in osteoporosis. Biomedical Reports, 10(1), 17-22.

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Osteoblast Differentiation and Quantification

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The cells were seeded in 6-well plates at a density of 0.8 × 10⁵ cells/well and stabilized for 24 h. To induce osteoblast differentiation, Osteogenic medium (Promocell), containing 50 μg/ml L-ascorbic acid and 10 mM β-glycerophosphate, was added to culture for 11 days. The culture medium was changed every 3–4 days. Then, the cells were fixed in 10% formalin for 10 min and stained with the 40 mM Alizarin Red-S (pH 4.2; Sigma-Aldrich; Merck KGaA) for 15 min, all at RT. For quantification of Alizarin red S, 500 μl citrate solution containing 20% methanol and 10% acetic acid was added for 20 min at RT, and the absorbance of supernatants was measured at 570 nm using a GloMAx Fluorescence Reader (Promega). The plates were observed under the Leica Microscope DML B2/11888111 equipped with Leica camera DFC450 at × 100 magnification.

Massaccesi L., Ragone V., Papini N., Goi G., Corsi Romanelli M.M, & Galliera E. (2019). Effects of Vitamin E-Stabilized Ultra High Molecular Weight Polyethylene on Oxidative Stress Response and Osteoimmunological Response in Human Osteoblast. Frontiers in Endocrinology, 10, 203.

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Quantitative Evaluation of Cardiac Remodeling

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For quantitative evaluation, three H&E- and Masson-stained sections per rat were used. By applying ImageJ software (NIH, Bethesda, MD, USA), the H&E- and Masson-stained sections were examined to determine the cardiomyocyte area and Masson’s-stained area, respectively. A Leica DMLB2/11888111 microscope equipped with a Leica DFC450 camera was used to acquire the images.
Connexin-43 immunofluorescence was measured by randomly capturing five non-overlapping images per slide. A Leica DM5500 B/11888817/12 microscope, fitted with a Leica HI PLAN 10×/0.25 objective and a Leica DFC450C camera, was used to capture the images. Connexin-43-stained spots were manually counted using the plugin/cell counting tool (Rangan & Tesch, 2007 (link)) in ImageJ software (National Institutes of Health, Bethesda, MD, USA), and the average per field for each rat was then calculated. This measurement was performed in a blind manner by an independent experienced researcher. For statistical analysis and comparison, ten animals were used per experimental group.

El-Akabawy G., El-Kersh S.O., El-Kersh A.O., Amin S.N., Rashed L.A., Abdel Latif N., Elshamey A., Abdallah M.A., Saleh I.G., Hein Z.M., El-Serafi I, & Eid N. (2024). Dental pulp stem cells ameliorate D-galactose-induced cardiac ageing in rats. PeerJ, 12, e17299.

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Quantitative Morphometric Assessment

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For morphometric assessment, nonoverlapping fields (×400) per section in three different serial sections from each rat were obtained using a Leica DML B2/11888111 microscope equipped with a Leica DFC450 camera. The examined parameters were calculated using ImageJ software version K1.45.

Khodir S.A., Faried M.A., Abd-Elhafiz H.I, & Sweed E.M. (2022). Sitagliptin Attenuates the Cognitive Deficits in L-Methionine-Induced Vascular Dementia in Rats. BioMed Research International, 2022, 7222590.

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Quantitative Assessment of Immunostaining

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For the quantitative assessment of immunostaining, three nonoverlapping images were randomly captured per section using a Leica DML B2/11888111 microscope equipped with a Leica DFC450 camera and Leica C PLAN 10 × 0.22 objective or a Leica DM5500 B/11888817/12 microscope equipped with a Leica DFC450C camera and Leica HI PLAN 10 × 0.25 objective. For each image, the field of view was at 10 × magnification. The number of immunopositive cells in the fields from at least three sections/animal was counted using ImageJ software (US National Institutes of Health, Bethesda, MD, USA) and averaged per field for each animal. This was blindly performed by an independent, experienced researcher. The calculated percentages for at least eight animals per experimental group were considered for comparison and statistical analyses.

Al-Serwi R.H., El-Kersh A.O, & El-Akabawy G. (2021). Human dental pulp stem cells attenuate streptozotocin-induced parotid gland injury in rats. Stem Cell Research & Therapy, 12, 577.

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Quantitative Analysis of Immunopositive Cells in the Cerebral Cortex and Basal Forebrain

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Five non-overlapping images per section were randomly captured from the cerebral cortex, whereas the entire basal forebrain and dentate gyral area were analysed for each brain section for each marker. Immunohistochemical images were captured using a Leica DML B2/11888111 microscope equipped with a Leica DFC450 camera, using the Leica C PLAN 4×/0.10 or 10×/0.22 objectives. Immunofluorescence images were captured using a Leica DM5500 B/11888817/12 microscope equipped with a Leica DFC450C camera, using the Leica HI PLAN 10×/0.25 objective. For each image, the region of interest was the field of view at a magnification of 10×. From at least three sections/rat, immunopositive cells were counted using the ImageJ software (National Institutes of Health, Bethesda, Maryland, US) by a manual approach using the plugin/cell counter tool [70] (link) and then averaged per field for each rat. Calculated numbers for 10 animals/experimental group were considered for comparison and statistical analyses.

El-Akabawy G., Aabed K., Rashed L.A., Amin S.N., AlSaati I, & Al-Fayez M. (2022). Preventive effects of bone marrow-derived mesenchymal stem cell transplantation in a D-galactose-induced brain aging in rats. Folia morphologica, 81(3).

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