Log In Sign up
The largest database of trusted experimental protocols

Mycoalert mycoplasma test kit

Manufactured by Lonza
Visit Supplier
Sourced in Switzerland

The MycoAlert mycoplasma test kit is a rapid, sensitive, and specific method for the detection of mycoplasma contamination in cell cultures. The kit utilizes an enzyme-based detection system to identify the presence of mycoplasma-specific enzymes in cell culture samples.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Mccoy s 5a media, by Thermo Fisher Scientific (1 mentions) Nuclightgreen, by Sartorius (1 mentions) Nuclightred, by Sartorius (1 mentions) Skov3 htb 77, by American Type Culture Collection (1 mentions) Puromycin, by PerkinElmer (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Bcp 1, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Mln0128, by Selleck Chemicals (1 mentions) Bcbl 1, by American Type Culture Collection (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Hek293t, by American Type Culture Collection (1 mentions)

5 protocols using mycoalert mycoplasma test kit

1

Establishment of Ovarian Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The SKOV-3 (HTB-77) ovarian cancer cell line was purchased from American Type Culture Collection (Manassas, Virginia, USA) and were maintained in McCoy’s 5A media (Gibco, Waltham, Massachusetts, USA) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1× pen–strep (Gibco). SKOV-3 cells stably expressing firefly luciferase (SKOV-3-Luc), NucLightGreen (NLG), or NucLightRed (NLR) (Sartorius, Göttingen, Germany) were generated as previously described.30 (link) The cancer cell lines OVCAR-4 (SCC258) and OVCAR-5 (SCC259) were purchased from MilliporeSigma (Burlington, Massachusetts, USA) and were maintained in Roswell Park Memorial Institute (RPMI)-1640 media (Gibco) supplemented with 10% FBS and 1× pen–strep. Cells were routinely tested for Mycoplasma with the MycoAlert Mycoplasma Test Kit (Lonza, Basel, Switzerland).
Snyder K.M., Dixon K.J., Davis Z., Hosking M., Hart G., Khaw M., Matson A., Bjordahl R., Hancock B., Shirinbak S., Miller J.S., Valamehr B., Wu J, & Walcheck B. (2023). iPSC-derived natural killer cells expressing the FcγR fusion CD64/16A can be armed with antibodies for multitumor antigen targeting. Journal for Immunotherapy of Cancer, 11(12), e007280.
+ Open protocol
+ Expand
2

Deriving Neural Precursor Cells from Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neural precursor cells (NPCs) were derived from H9 and HUES9 human
embryonic stem cells (Supplementary Fig. 3). We used primary human foreskin fibroblasts
(HFFs), as in Belzile et al. J Virol. 2014 Apr;88(8):4021-39 30 (link), and Endothelial cells (ECs)
as in DuRose et al. J Virol. 2012 Dec;86(24):13745-55 6 (link). Cells were routinely tested for mycoplasma
contamination using the MycoAlert mycoplasma test kit (Lonza) and were found
negative for mycoplasma.
Batra R., Stark T.J., Clark A.E., Belzile J.P., Wheeler E.C., Yee B.A., Huang H., Gelboin-Burkhart C., Huelga S.C., Aigner S., Roberts B.T., Bos T.J., Sathe S., Donohue J.P., Rigo F., Ares M J.r., Spector D.H, & Yeo G.W. (2016). RNA binding protein CPEB1 remodels host and viral RNA landscapes. Nature structural & molecular biology, 23(12), 1101-1110.
+ Open protocol
+ Expand
3

Evaluation of Rapamycin and MLN0128 Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols
MLN0128 and rapamycin were purchased from Selleckchem. Compounds were dissolved in DMSO (Sigma-Aldrich), aliquoted, and stored at −80°C. Cell lines were cultured in RPMI 1640 supplemented with 100 U/ml penicillin-streptomycin (Thermo Fisher), 2 mM l-glutamine (Thermo Fisher), and 10% fetal bovine serum (FBS) (Sigma-Aldrich), maintained at 37°C in 5% CO2, and passaged for no more than 3 months. BC-1, BCBL-1, BC-3, and BCP-1 were obtained from ATCC. BCBL-1TrexRTA cells were a gift from J. Jung (46 (link)). RedFect (Perkin Elmer) was used to confer continuous red-luciferase expression (BCBL-1TrexRTA-luc), and cells were maintained in hygromycin B (20 µg/ml) and puromycin (1.25 µg/ml). Cell lines were authenticated by NextGen-based HLA and short tandem repeat (STR) typing (https://www.med.unc.edu/vironomics) and found free of mycoplasma by the Mycoalert mycoplasma test kit (Lonza).
Caro-Vegas C., Bailey A., Bigi R., Damania B, & Dittmer D.P. (2019). Targeting mTOR with MLN0128 Overcomes Rapamycin and Chemoresistant Primary Effusion Lymphoma. mBio, 10(1), e02871-18.
+ Open protocol
+ Expand
4

Cell Line Authentication and Mycoplasma Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T, HEK293XT and HeLa cells were purchased from the American Type Culture Collection and were not further authenticated. Cells were routinely tested for mycoplasma contamination with a MycoAlert mycoplasma test kit (Lonza) and were found negative for mycoplasma.
Luo E.C., Nathanson J.L., Tan F.E., Schwartz J.L., Schmok J.C., Shankar A., Markmiller S., Yee B.A., Sathe S., Pratt G.A., Scaletta D.B., Ha Y., Hill D.E., Aigner S, & Yeo G.W. (2020). Large-scale tethered function assays identify factors that regulate mRNA stability and translation. Nature structural & molecular biology, 27(10), 989-1000.
+ Open protocol
+ Expand
5

Deriving Neural Precursor Cells from Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neural precursor cells (NPCs) were derived from H9 and HUES9 human
embryonic stem cells (Supplementary Fig. 3). We used primary human foreskin fibroblasts
(HFFs), as in Belzile et al. J Virol. 2014 Apr;88(8):4021-39 30 (link), and Endothelial cells (ECs)
as in DuRose et al. J Virol. 2012 Dec;86(24):13745-55 6 (link). Cells were routinely tested for mycoplasma
contamination using the MycoAlert mycoplasma test kit (Lonza) and were found
negative for mycoplasma.
Batra R., Stark T.J., Clark A.E., Belzile J.P., Wheeler E.C., Yee B.A., Huang H., Gelboin-Burkhart C., Huelga S.C., Aigner S., Roberts B.T., Bos T.J., Sathe S., Donohue J.P., Rigo F., Ares M J.r., Spector D.H, & Yeo G.W. (2016). RNA binding protein CPEB1 remodels host and viral RNA landscapes. Nature structural & molecular biology, 23(12), 1101-1110.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!