Ea 1110 chns

Chemicals and solvents were provided from the research center Taros Chemicals GmbH & Co. KG, Dortmund, Germany or purchased from Sigma-Aldrich (Milan, Italy). Mps: Buchi apparatus, uncorrected. ¹HNMR were recorded on a Bruker Advance spectrometer operating at 300 MHz or on a Varian Gemini-200 instrument at 200 MHz; chemical shifts are given in ppm (δ) from tetramethylsilane as an internal standard, coupling constant(s) (J) in hertz (Hz). Elemental analyses were performed on a Carlo Erba EA-1110 CHNS instrument in the Microanalysis Laboratory of the Department of Pharmacy of Genoa University. Analytical, preparative HPLC and Electron Spray Ionization condition (ESI) mass spectra were performed on an Agilent uHPLC (1290 Infinity) and an Agilent Prep-HPLC (1260 Infinity) both equipped with a Diode Array Detector and a Quadrupole MSD using mixture gradients of formic acid/water/acetonitrile as system solvent.

Chemicals, solvents and commercially available compounds [Proguanil (30), 4-chlorophenylbiguanide (31), pyrimetamine (32) and trimethoprim (33)] were purchased from Sigma-Aldrich (Milan, Italy). Mps: Büchi apparatus, uncorrected. ¹H NMR and ¹³C NMR spectra were recorded on a Varian Gemini-200 instrument at 200 and 50 MHz, respectively; DMSO-d₆; δ in ppm rel. to Me₄Si as internal standard. J in Hz. Elemental analyses were performed on a Carlo Erba EA-1110 CHNS instrument in the Microanalysis Laboratory of the Department of Pharmacy of Genoa University.