Ovarian cancer A2780 and Iose386 cell lines were seeded into 6- well plates and grown to reach approximately 80% confluency. Next, cells were fixed in 4% paraformaldehyde and subsequently blocked in 5% non-fat milk for 1 h and then with the ROR1-cFab for 1 h at the room temperature and further with a goat anti-human IgG (Fab specific)-FITC antibody (Sigma Chemicals, St. Louis, MO, USA) for 1 h. 4′-6-diamidino-2-phenylindole (DAPI, Biotium, Hayward, CA, USA) was used to stain cell nuclei. Cells were analyzed under a fluorescence microscope (Zeiss, Jena, Germany).
Goat anti human igg fab specific fitc antibody
Manufactured by Merck Group
Sourced in United States
The Goat anti-human IgG (Fab specific)-FITC antibody is a laboratory reagent used for the detection and analysis of human immunoglobulin G (IgG) molecules. The antibody specifically binds to the Fab region of human IgG, and is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate) for visualization and quantification purposes.
Automatically generated - may contain errors
3 protocols using goat anti human igg fab specific fitc antibody
1
Immunofluorescence Imaging of Ovarian Cancer Cells
2
Quantifying Fab03 Binding on THP-1 Macrophages
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The THP-1-derived macrophages were treated with the hS9-Fab03 for 2 h; the cells were then incubated with goat anti-human IgG (Fab specific)-FITC antibody (Sigma-Aldrich, MO, USA) at 37°C for 1 h. The cells were analyzed by LSRII flow cytometer (BD Biosciences, CA, USA).
3
ROR1-cFab Binding in Ovarian Cancer
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Ovarian cancer A2780 and Iose386 cell lines were incubated with the ROR1-cFab for 1h and then incubated with a goat anti-human IgG (Fab specific)-FITC antibody (Sigma Chemicals, St. Louis, MO, USA) for 1 h. After being washed in PBS, the fluorescence intensity of cells was detected by the LSRII flow cytometer (BD Biosciences, San Jose, CA, USA).
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