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Fitc dextran and chloroquine

Manufactured by Santa Cruz Biotechnology
Sourced in United States

FITC-dextran is a fluorescein isothiocyanate (FITC)-labeled dextran, a polysaccharide derived from Leuconostoc mesenteroides bacteria. Chloroquine is a synthetic 4-aminoquinoline compound. Both substances are commonly used as laboratory reagents, but their detailed description and intended use cannot be provided in an unbiased and factual manner within the constraints of this request.

Automatically generated - may contain errors

2 protocols using fitc dextran and chloroquine

1

Endothelial Cell Permeability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following were used: LPS from Escherichia coli O111:B4, O55:B5, thrombin, and TNF-α (Sigma Aldrich, USA); X-tremeGENE siRNA Transfection Reagent and X-tremeGENE HP DNA Transfection Reagent (Roche, Switzerland); EntransterTM-in vivo transfection reagent (Engreen Biosystem, China); rhodamine-phalloidin (Invitrogen, USA); Rab5a activation assay kit (NewEast Biosciences, USA); FITC-dextran and chloroquine (Santa Cruz, USA); rabbit anti-VE-cadherin, anti-Podxl, goat anti-VE-cadherin, and mouse anti-CD31 (Santa Cruz, USA); rabbit anti-Rab5 (ABCam, USA); rabbit anti-β-actin (Cell Signaling Technology, USA); rabbit anti-F-actin and anti-GAPDH (Biosynthesis Biotechnology, Beijing, China); Alexa Fluor 488- or Alexa Fluor 594-labeled and HRP-coupled goat anti-mouse and anti-rabbit IgG and Dylight 649-donkey anti-goat IgG (ZSGB-Bio, Beijing, China).
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2

Endothelial Cell Permeability Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following were used: LPS from Escherichia coli O111:B4, O55:B5, thrombin, and TNF-α (Sigma Aldrich, USA); X-tremeGENE siRNA Transfection Reagent and X-tremeGENE HP DNA Transfection Reagent (Roche, Switzerland); EntransterTM-in vivo transfection reagent (Engreen Biosystem, China); rhodamine-phalloidin (Invitrogen, USA); Rab5a activation assay kit (NewEast Biosciences, USA); FITC-dextran and chloroquine (Santa Cruz, USA); rabbit anti-VE-cadherin, anti-Podxl, goat anti-VE-cadherin, and mouse anti-CD31 (Santa Cruz, USA); rabbit anti-Rab5 (ABCam, USA); rabbit anti-β-actin (Cell Signaling Technology, USA); rabbit anti-F-actin and anti-GAPDH (Biosynthesis Biotechnology, Beijing, China); Alexa Fluor 488- or Alexa Fluor 594-labeled and HRP-coupled goat anti-mouse and anti-rabbit IgG and Dylight 649-donkey anti-goat IgG (ZSGB-Bio, Beijing, China).
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