Plasma samples were assayed using a Human Cytokine Milliplex Map kit (Millipore Corporation, Billerica, MA, USA). Measurements were performed using a Bio-Plex® 200 SystemsReader (Bio-Rad Laboratories, Hercules, CA, USA).
Bio plex 200 systemsreader
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex® 200 Systems Reader is a multiplex analysis instrument that uses fluorescent-dyed magnetic beads to quantify multiple analytes simultaneously in a single sample. The system can detect and measure up to 100 different analytes in a single well. The reader employs flow cytometry technology to analyze the beads.
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Lab products found in correlation
2 protocols using bio plex 200 systemsreader
1
Plasma Cytokine Profiling Assay
2
Quantification of Inflammatory Markers
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Levels of IL-1β, IL-6, IL-8, S100A8/A9, and galectin-3 were determined using Human ProcartaPlex Multiplex kits (ThermoFisher Scientific) following the instructions provided by the manufacturer. Briefly, following 24 h treatment with toxins or the positive control LPS (10 ng/ml), cell supernatants were transferred to 96-well microplates and stored at -80 °C until further analysis. After accumulation of the magnetic bead solution to 96-well flat bottom plate, samples were added and incubated overnight. Following washing steps, detection antibody mix was incubated for 30 minutes on a plate shaker at 500 rpm. After washing, streptavidin peroxidase was added for 30 min on a plate shaker at 500 rpm. After a final washing step, reading buffer was added and the plate was analyzed using a Bio-Plex 200 Systems reader (Bio-Rad).
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