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Zen zen lite imaging software

Manufactured by Zeiss

The ZEN/ZEN lite imaging software is a comprehensive suite of tools developed by Zeiss for the acquisition, processing, and analysis of microscopic images. It provides a user-friendly interface and a range of advanced features to support researchers and scientists in their workflows.

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4 protocols using zen zen lite imaging software

1

Live-cell Imaging of Transfected HeLa Cells

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HeLa cells incubated with various siRNA oligos were plated in dishes with glass base (Thermo Scientific #150682) in DMEM medium supplemented with 10% FBS overnight before transient transfection of GFP-tagged cDNAs of indicated genes with Lipofactemine®3000 reagent. One-hour post-transfection protocols the cells were incubated in fresh medium and incubated for sixteen hours at 37°C supplemented with 5% CO2. Live cell imaging of cells was carried out with Zeiss LSM-510 META confocal microscopes using 63× oil-based objective in incubation chamber at 37°C supplemented with 5% CO2. In each experiment 10 independent frames were captured. Images were captured and processed with ZEN/ZEN lite imaging software from Zeiss.
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2

Imaging of GFP-FBXL2 in HeLa Cells

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HeLa cells were grown in dishes with a glass base (Thermo Scientific no. 150682) in DMEM medium supplemented with 10% FBS. Cells were transfected with GFP-FBXL2 cDNA using Lipofactemine 3000 reagent. Two hours post-transfection, cells were incubated in fresh medium containing GGTi-2418 (15 μM) for 16 h at 37 °C supplemented with 5% CO2. Live-cell imaging was carried out with a Zeiss LSM-510 META confocal microscope using a 63× oil-based objective in an incubation chamber at 37 °C supplemented with 5% CO2. Images were captured and processed with ZEN/ZEN lite imaging software from Zeiss.
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3

Imaging of GFP-FBXL2 in HeLa Cells

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HeLa cells were grown in dishes with a glass base (Thermo Scientific no. 150682) in DMEM medium supplemented with 10% FBS. Cells were transfected with GFP-FBXL2 cDNA using Lipofactemine 3000 reagent. Two hours post-transfection, cells were incubated in fresh medium containing GGTi-2418 (15 μM) for 16 h at 37 °C supplemented with 5% CO2. Live-cell imaging was carried out with a Zeiss LSM-510 META confocal microscope using a 63× oil-based objective in an incubation chamber at 37 °C supplemented with 5% CO2. Images were captured and processed with ZEN/ZEN lite imaging software from Zeiss.
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4

Live-cell Imaging of Transfected HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells incubated with various siRNA oligos were plated in dishes with glass base (Thermo Scientific #150682) in DMEM medium supplemented with 10% FBS overnight before transient transfection of GFP-tagged cDNAs of indicated genes with Lipofactemine®3000 reagent. One-hour post-transfection protocols the cells were incubated in fresh medium and incubated for sixteen hours at 37°C supplemented with 5% CO2. Live cell imaging of cells was carried out with Zeiss LSM-510 META confocal microscopes using 63× oil-based objective in incubation chamber at 37°C supplemented with 5% CO2. In each experiment 10 independent frames were captured. Images were captured and processed with ZEN/ZEN lite imaging software from Zeiss.
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