Anti hp1β

The following primary antibodies were used in this study: anti-H3K9me3 (Abcam, cat# ab8898; IF 1:1000, WB 1:2000), anti-H3K9me2 (Active Motif, cat# 39239, IF 1:1000), anti-Lamin A/C (Santa Cruz, cat# sc-376248; IF 1:500), anti-Lamin B1 (Abcam, cat# ab16048; IF 1:1000), anti-GFP (Abcam, cat# ab290; WB 1:1000, IP 1:200), Normal Rabbit IgG (Cell Signaling, cat# 2729; IP 1:200), anti-HP1α (Abcam, cat# 77256; IF, WB 1:1000), anti-HP1β (Abcam, cat# ab10478; IF, WB 1:1000), anti-HP1γ (Santa Cruz, cat# sc-398562; IF 1:500, WB 1:1000), anti-PRR14 (Proteintech, cat# 22819-1-AP; WB 1:1000), anti-β-Actin (8H10D10) (Cell Signaling, cat# 3700S; WB 1:5000), anti-Histone H3 (Abcam, cat# ab1791; WB 1:10,000), and anti-GAPDH (D4C6R) (Cell Signaling, cat# 51332; WB 1:1000).
The following secondary antibodies from ThermoFisher Scientific/Invitrogen were used: Donkey anti-Rabbit Alexa Fluor 568 (cat# A10042, IF 1:1000), Donkey anti-Mouse Alexa Fluor 488 (cat# A10037, IF 1:1000), Donkey anti-Rabbit Alexa Fluor 647 (cat# A31573, IF 1:1000), Donkey anti-Mouse Alexa Fluor 647 (cat# A31571, IF 1:1000); and HRP-linked anti-Rabbit IgG and HRP-linked anti-Mouse IgG (Cell Signaling, cat# 7074 and 7076; WB 1:5,000). IF – immunofluorescence, WB – Western Blot, IP – immunoprecipitation.

Primary antibodies used for western blot, including the polyclonal rabbit anti-H3 (Cat # ab1791), anti-H3K9me3 (Cat # ab8898), and anti-HP1β (Cat #10478) antibodies, were purchased from Abcam and the secondary antibody, anti-rabbit-IgG AF647 (Cat # A32733), from Invitrogen. The primary mouse monoclonal anti-H1 antibody (H-2) was purchased from Santa Cruz Biotechnology (Cat # sc-393358) and the secondary antibodies, anti-mouse IgG-HRP (Cat # A9044), and anti-rabbit IgG-HRP (Cat # A6154) were purchased from Sigma-Aldrich.