The phenotype of CIK cells was detected by a multiparameter flow cytometric analysis using monoclonal antibodies: anti-CD3-FITC, anti-CD56-PE, anti-CD4-APC, anti-CD8a-Brilliant Violet 421, anti-PD-1-APC and anti-CTLA-4-APC (obtained from Biolegend, San Diego, CA, U.S.A.) and 7AAD (a viability probe for methods of dead cell exclusion, BD biosciences, San Diego, CA, U.S.A.). To observe the surface expression of PD-L1/PD-L2 on RCC cell lines, we stained with monoclonal antibodies anti-PD-L1-PE and anti-PD-L2-Brilliant Violet 421 (obtained from Biolegend, San Diego, CA, U.S.A.) and 7AAD.
Anti ctla 4 apc
Manufactured by BioLegend
Sourced in United States
Anti-CTLA-4-APC is a monoclonal antibody that binds to the CTLA-4 protein expressed on the surface of T cells. It is conjugated with the fluorescent dye allophycocyanin (APC) for flow cytometric detection and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 fitc, by BioLegend (1 mentions)
Anti cd4 apc, by BioLegend (1 mentions)
Anti pd l1 pe, by BioLegend (1 mentions)
Anti cd56 pe, by BioLegend (1 mentions)
Anti pd 1 apc, by BioLegend (1 mentions)
Anti pd 1 pe, by Thermo Fisher Scientific (1 mentions)
Anti pd l1 pe, by Thermo Fisher Scientific (1 mentions)
Kaluza 1, by Beckman Coulter (1 mentions)
Anti cd3 pe, by Thermo Fisher Scientific (1 mentions)
Gallios flow cytometer, by Beckman Coulter (1 mentions)
2 protocols using anti ctla 4 apc
1
CIK Cell Phenotyping and RCC PD-L Expression
2
Exosome Protein Detection by Flow Cytometry
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The on-bead flow cytometry was used for detection of proteins carried by exosomes as previously reported20 -22 and described in Supplemental Methods . The following detection Abs were used: anti-PD-L1 PE (12–5983-42, e-Bioscience) or anti-PD-1 PE (12–2799-42, e-Bioscience), anti-CTLA4-APC (349908, Biolegend), anti-CD15s-PE (sc-32243, Santa Cruz), anti-CD3-PE (12–0037-42, e-Bioscience). Isotype control Abs were used in all experiments.
Detection of exosome cargo components was performed using the Gallios flow cytometer equipped with Kaluza 1.0 software (Beckman Coulter, Krefeld, Germany). Samples were run for 2 min and around 10,000 events were acquired. Gates were set on the bead fraction visible in the forward/sideward light scatter. Reproducibility of this flow cytometry-based detection assay was established as described inSupplemental Methods . Flow cytometry results of a representative experiment are shown in Supplemental Figure 3.
Detection of exosome cargo components was performed using the Gallios flow cytometer equipped with Kaluza 1.0 software (Beckman Coulter, Krefeld, Germany). Samples were run for 2 min and around 10,000 events were acquired. Gates were set on the bead fraction visible in the forward/sideward light scatter. Reproducibility of this flow cytometry-based detection assay was established as described in
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