Monna

Manufactured by Merck Group

MONNA is a high-precision laboratory equipment designed for accurate and reliable measurements. It serves as a versatile tool for various applications in scientific research and analysis.

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Lab products found in correlation

T16ainh a01, by Merck Group (3 mentions) Tannic acid, by Merck Group (2 mentions) Cftrinh 172, by Merck Group (2 mentions) Caccinh a01, by Merck Group (2 mentions) Recombinant human il 4, by R&D Systems (1 mentions) Amiloride, by Merck Group (1 mentions) Niflumic acid, by Merck Group (1 mentions) Ribavirin, by Merck Group (1 mentions) Glibenclamide, by Bio-Techne (1 mentions) R iaa94, by Merck Group (1 mentions) Incucyte zoom imager, by Sartorius (1 mentions) Chromanol 293b, by Merck Group (1 mentions)

3 protocols using monna

High-throughput CFTR Inhibitor Screening

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T16A_inh-A01, MONNA, CFTR_inh-172, amiloride, tannic acid and other chemicals, unless otherwise indicated, were purchased from Sigma-Aldrich (St. Louis, MO). Ani9 and its analogs were purchased from ChemDiv (San Diego, CA). Recombinant Human IL-4 was purchased from R&D systems (Minneapolis, MN). The compound collections used for screening included 54,400 drug-like molecules were purchased from ChemDiv. The compounds were diluted with DMSO to reach a concentration of 2.5 mM. This was used as the 100x concentrated stock solution.

Seo Y., Lee H.K., Park J., Jeon D.K., Jo S., Jo M, & Namkung W. (2016). Ani9, A Novel Potent Small-Molecule ANO1 Inhibitor with Negligible Effect on ANO2. PLoS ONE, 11(5), e0155771.

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Screening and Characterization of Novel Antagonists

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CaCCinh-A01, MONNA, and T16Ainh-A01 were purchased from Sigma-Aldrich and tizoxanide was purchased from J&W Pharmlab LLC. Niclosamide (Cpd 1) and niclosamide-related compounds Cpd 2, Cpd 3, Cpd 4, and Cpd 6 – 18, nitazoxanide and CID# 2806957 were from the Amgen small molecule compound collection, as were the benchmark antagonists niflumic acid, dichlorophen, benzbromarone, 1PBC and NTTP. Cpd 5 was synthesized according to a literature procedure (Macielag et al., 1998 (link)). The source for other compounds and reagents is provided in each experimental section.

Miner K., Labitzke K., Liu B., Wang P., Henckels K., Gaida K., Elliott R., Chen J.J., Liu L., Leith A., Trueblood E., Hensley K., Xia X.Z., Homann O., Bennett B., Fiorino M., Whoriskey J., Yu G., Escobar S., Wong M., Born T.L., Budelsky A., Comeau M., Smith D., Phillips J., Johnston J.A., McGivern J.G., Weikl K., Powers D., Kunzelmann K., Mohn D., Hochheimer A, & Sullivan J.K. (2019). Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide Are Potent TMEM16A Antagonists That Fully Bronchodilate Airways. Frontiers in Pharmacology, 10, 51.

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HRSV Infection Inhibition by Chloride Channel Modulators

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A549 or SH-SY5Y cells (1×10⁴ cells/well) in 96 wells were pretreated with the indicated Cl⁻ channel blockers and infected with HRSV-GFP (MOI: 0.1) in the presence of each inhibitor for 24 hours. HRSV-GFP fluorescence was visualised using an IncuCyte ZOOM imager (IncuCyte, Essen Bioscience). Fluorescence was quantified using accompanying analysis software. The Cl⁻ channel modulators used in the study were as follows: broad-spectrum Cl⁻ channel inhibitors 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB—Sigma-Aldrich, N4779), 4,4ʹ-diisothiocyanostilbene-2,2ʹ-disulfonic acid (DIDS—Sigma-Aldrich, D3514) and R(+)-[(6,7-Dichloro-2-cyclopentyl-2,3-dihydro-2-methyl-1-oxo-1H-inden-5-yl)-oxyacetic acid (R+IAA-94—Sigma-Aldrich, I117); CFTR inhibitors: CFTRinh-172 (Sigma-Aldrich, C2992), chromanol 293B (Sigma-Aldrich, C2615) and glibenclamide (Tocris Biosciences, 0911); CaCC inhibitors: CaCCinh-A01 (Sigma-Aldrich, SML0916), niflumic acid (Sigma-Aldrich, N0630), talniflumate (Sigma-Aldrich, SML1710) and tannic acid (Sigma-Aldrich, 403040); TMEM16A inhibitors: T16Ainh-A01 (Merck Chemicals, 613551) and MONNA (Sigma-Aldrich, SML0902). Ribavirin (Sigma-Aldrich, R9644) was included as a known HRSV inhibitor.

Pearson H., Todd E.J., Ahrends M., Hover S.E., Whitehouse A., Stacey M., Lippiat J.D., Wilkens L., Fieguth H.G., Danov O., Hesse C., Barr J.N, & Mankouri J. (2020). TMEM16A/ANO1 calcium-activated chloride channel as a novel target for the treatment of human respiratory syncytial virus infection. Thorax, 76(1), 64-72.

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