Procartaplex multiplex immunoassays panels

Serum levels of vascular endothelial growth factor A (VEGF-A), P-selectin, E-selectin, platelet endothelial cell adhesion molecular (PECAM-1), CD40 ligand (CD40L), tissue plasminogen activator (tPA), plasminogen activator inhibitor 1 (PAI-1), serum amyloid antigen 1 (SAA-1), vascular cell adhesion molecular 1 (VCAM-1) and intercellular adhesion molecular 1 (ICAM-1) were determined by the ProcartaPlex multiplex immunoassays panels (Affymetrix, USA) according to the manufacturer instructions. Each serum sample was tested in duplicates and the concentration was further determined according to the dilution curve of standard materials.

For all the hospitalized patients, the serum samples had been collected, stored at -80°C and archived into the biological bank, which were used for the current cytokine assay. Briefly, the cytokine/chemokines were evaluated from the acute serum samples by the Bio-plex Pro Human 27-plex cytokine panel (Bio-Rad) following manufacturer’s instruction. Serum levels of vascular endothelial growth factor A (VEGF-A), P-selectin, E-selectin, platelet endothelial cell adhesion molecular (PECAM-1), CD40 ligand (CD40L), tissue plasminogen activator (tPA), plasminogen activator inhibitor 1 (PAI-1), serum amyloid antigen 1 (SAA-1), vascular cell adhesion molecular 1 (VCAM-1) and intercellular adhesion molecular 1 (ICAM-1) were determined by the ProcartaPlex multiplex immunoassays panels (Affymetrix, USA) according to the manufacturer instructions.

Laboratory-confirmed SFTS patients with positive SFTSV RNA detection by qRT-PCR were recruited from the PLA 990 hospital, Xinyang in Henan province. Sera were collected at the acute and late phases of illness. Data about demography, clinical features, laboratory data, and outcome, were retrospectively collected. Healthy individuals that were comparable in age and sex were recruited as the control group. Informed consent was obtained from all individuals. The study was approved by the Ethical Committee of the Beijing Institute of Microbiology and Epidemiology.
Levels of chymase and tryptase in the serum were tested by commercial ELISA kits (Abbexa, UK and Cloud-Clone Corp., China). A series of cytokines and adhesion molecules related to vascular endothelial dysfunction, including platelet endothelial cell adhesion molecular (PECAM-1), vascular endothelial growth factor A (VEGF-A), serum amyloid antigen 1 (SAA-1), vascular cell adhesion molecular 1 (VCAM-1), intercellular adhesion molecular 1 (ICAM-1), P-selectin, E-selectin, tissue plasminogen activator (tPA) and plasminogen activator inhibitor 1 (PAI-1) were tested in the serum by the ProcartaPlex multiplex immunoassays panels (Affymetrix, USA).