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Donor calf serum

Manufactured by Thermo Fisher Scientific
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Sourced in France

Donor calf serum is a cell culture supplement derived from the blood of young calves. It provides a complex mixture of proteins, growth factors, and other nutrients that support the growth and maintenance of cells in vitro.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (4 mentions) Putrescin, by Merck Group (2 mentions) Insulin, by Merck Group (2 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Mda mb 468, by American Type Culture Collection (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Click it plus kit, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions)

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Calf serum

4 protocols using donor calf serum

1

Immortalized Mouse Embryo Fibroblasts and Triple-Negative Breast Cancer Cell Lines

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Spontaneously immortalized mouse embryo fibroblasts (MEFs) were obtained from Rodent transgenic core facility at Cedars-Sinai and maintained in DMEM (Invitrogen, Carlsbad, CA) supplemented with 10% donor calf serum (Invitrogen) and 100U/mL penicillin and 100 mg/mL streptomycin.
Human triple-negative breast cancer cell lines MDA-MB-231 and MDA-MB-468 were obtained from ATCC. These TNBC cells are highly proliferative, lack the expression of hormone receptors ER (estrogen receptor) and PR (progesterone receptor), and do not overexpress the Her2 receptor. Cells were grown in the Roswell Park Memorial Institute (RPMI)-1640 (Mediatech, Manassas, VA, USA) supplemented with 10% donor calf serum (Invitrogen), 100U/mL penicillin and 100 mg/mL streptomycin (Invitrogen). Both cells were incubated at 37 °C in a humidified atmosphere containing 5% CO2. CRL1101 was purchased from Chembridge, Inc. (San Diego, CA).
Kanzaki H., Chatterjee A., Hossein Nejad Ariani H., Zhang X., Chung S., Deng N., Ramanujan V.K., Cui X., Greene M.I, & Murali R. (2021). Disabling the Nuclear Translocalization of RelA/NF-κB by a Small Molecule Inhibits Triple-Negative Breast Cancer Growth. Breast Cancer : Targets and Therapy, 13, 419-430.
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2

Isolation and Culture of Rat Tanycytes

Cited 1 time
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Tanycytes were isolated from the median eminence of the hypothalamus of 10-d-old rats and cultured as described previously 54 (link),84 (link). Briefly, after decapitation and removal of the brain, median eminences were dissected and crushed on 80μM nylon mesh (Sefar America Inc., Kansas City, MO). Dissociated cells were cultured in DMEM/F12 (Invitrogen, Cergy Pontoise, France) supplemented with 10% (v/v) donor calf serum (Invitrogen) under humid atmosphere of 5 % CO2-95 % air at 37 °C. Culture medium was changed after 3-4 days of culture and subsequently every 2 days. Upon reaching confluence, the tanycytes were isolated from contaminating cells by overnight shaking at 250 rpm at 37 °C and either replated in 6 cm dishes for Western blot experiments or seeded in culture plates on poly-L-lysine-coated glass coverslips for studying leptin traficking. Two days before treatment, the medium was replaced by a tanycyte defined medium (TDM) consisting of DMEM/F12 (devoid of phenol red; Invitrogen) supplemented with insulin (5μg/ml) (Sigma, Saint Quentin Fallavier, France) and putrescin (100μM) (Sigma).
Duquenne M., Folgueira C., Bourouh C., Millet M., Silva A., Clasadonte J., Imbernon M., Fernandois D., Martinez-Corral I., Kusumakshi S., Caron E., Rasika S., Deliglia E., Jouy N., Oishi A., Mazzone M., Trinquet E., Tavernier J., Kim Y.B., Ory S., Jockers R., Schwaninger M., Boehm U., Nogueiras R., Annicotte J.S., Gasman S., Dam J, & Prévot V. (2021). Leptin brain entry via a tanycytic LepR:EGFR shuttle controls lipid metabolism and pancreas function. Nature metabolism, 3(8), 1071-1090.
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3

Isolation and Transduction of Rat Tanycytes

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Tanycytes were isolated from 10 days old rat as previously described69 (link) and cultured in DMEM/F12 (31966, Thermo Fisher Scientific) supplemented with 10% (v/v) donor calf serum (Invitrogen), 1% (v/v) L-glutamine (Thermo Fisher Scientific), and 2% penicillin/streptomycin. The tanycytes were plated on inserts (Grenier bio-one 665641) at a density of 442 cells/mm2 and were transduced the same day with the PKCαWT (MOI 10) and PKCαD463H (MOI 20) lentiviruses in the presence of 1 μg/ml of polybrene. The following day the medium was changed. Two days post transduction EdU (1.6 μg/ml) was added to the medium; 12 h later (day 3 post transduction) the cells were fixed and processed for immunostaining as described for astrocytes. EdU staining was chemically revealed with the Click-iT plus kit (C10640, Thermo Fisher Scientific).
Rosenberg S., Simeonova I., Bielle F., Verreault M., Bance B., Le Roux I., Daniau M., Nadaradjane A., Gleize V., Paris S., Marie Y., Giry M., Polivka M., Figarella-Branger D., Aubriot-Lorton M.H., Villa C., Vasiljevic A., Lechapt-Zalcman E., Kalamarides M., Sharif A., Mokhtari K., Pagnotta S.M., Iavarone A., Lasorella A., Huillard E, & Sanson M. (2018). A recurrent point mutation in PRKCA is a hallmark of chordoid gliomas. Nature Communications, 9, 2371.
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4

Isolation and Culture of Rat Tanycytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tanycytes were isolated from the median eminence of the hypothalamus of 10-d-old rats and cultured as described previously 54 (link),84 (link). Briefly, after decapitation and removal of the brain, median eminences were dissected and crushed on 80μM nylon mesh (Sefar America Inc., Kansas City, MO). Dissociated cells were cultured in DMEM/F12 (Invitrogen, Cergy Pontoise, France) supplemented with 10% (v/v) donor calf serum (Invitrogen) under humid atmosphere of 5 % CO2-95 % air at 37 °C. Culture medium was changed after 3-4 days of culture and subsequently every 2 days. Upon reaching confluence, the tanycytes were isolated from contaminating cells by overnight shaking at 250 rpm at 37 °C and either replated in 6 cm dishes for Western blot experiments or seeded in culture plates on poly-L-lysine-coated glass coverslips for studying leptin traficking. Two days before treatment, the medium was replaced by a tanycyte defined medium (TDM) consisting of DMEM/F12 (devoid of phenol red; Invitrogen) supplemented with insulin (5μg/ml) (Sigma, Saint Quentin Fallavier, France) and putrescin (100μM) (Sigma).
Duquenne M., Folgueira C., Bourouh C., Millet M., Silva A., Clasadonte J., Imbernon M., Fernandois D., Martinez-Corral I., Kusumakshi S., Caron E., Rasika S., Deliglia E., Jouy N., Oishi A., Mazzone M., Trinquet E., Tavernier J., Kim Y.B., Ory S., Jockers R., Schwaninger M., Boehm U., Nogueiras R., Annicotte J.S., Gasman S., Dam J, & Prévot V. (2021). Leptin brain entry via a tanycytic LepR:EGFR shuttle controls lipid metabolism and pancreas function. Nature metabolism, 3(8), 1071-1090.
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