The serum ADMA concentration was determined using an enzyme-linked immunosorbent assay kit (DLD Diagnostika GmbH, Hamburg, Germany).17 (link),18 (link) The serum concentration of NOx was assessed using the Griess method with an NOx colorimetric assay kit (Cayman Chemical, Ann Arbor, MI, USA). The plasma concentrations of amino acids involved in citrulline–arginine recycling (Figure 1 )13 (link),14 (link) were determined using routine ion-exchange chromatography with an autoanalyzer (L822; Hitachi High-Technologies Corp., Tokyo, Japan).
Nox colorimetric assay kit
Manufactured by Cayman Chemical
Sourced in United States
The NOx colorimetric assay kit is a laboratory tool designed to measure the concentration of nitric oxide (NO) and nitrite (NO2) in biological samples. The kit utilizes a colorimetric method to quantify the total amount of NO and NO2 present in the sample. The kit provides a simple and reliable way to assess the levels of these important signaling molecules in various research applications.
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Lab products found in correlation
2 protocols using nox colorimetric assay kit
1
Serum ADMA, NOx, and Amino Acid Levels Analysis
2
Bladder Function and Nitric Oxide
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At the end of week 11, 12 rats from each group were placed in individual 3701M081 metabolic cages (Tecniplast, Buguggiate, Italy) equipped with an FT-104 force transducer (iWorx/CB Sciences, Inc., Dover, NH, USA), as reported previously33 (link). After a familiarisation period (24 h), the volume of liquid consumed, micturition frequency, and urine output were measured for 3 days, and an average value was determined. Subsequently, the OGTT was performed after an overnight fast33 (link). On the next day, we collected the urine of the rats before and 2 h after subcutaneous insulin injection (1 U/kg). NO oxidation was measured by the use of a NOx Colorimetric Assay kit (Cayman). The level of urinary NOx was considered as an estimate of bladder NO availability.
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