Plant material (aerial parts, 250 g) carefully selected and dried was subjected to hydro distillation method for three hours, using an all glass Clevenger-type apparatus according to the well-known methods described in the literature (10 ). The final product obtained was dried and stored at −20°C until further analysis.
In order to obtain summer savory total TE Soxhlet extraction method was employed. Aerial parts (50 g), crushed and homogenized, were placed in the Soxhlet apparatus (Solvent Extractor™, SER 148 Series; Velp Scientifica, Usmate, Italy) and 400 ml of 70% EtOH were used to sequentially extraction for 48 h. The final extract was filtered through filter paper, the solvent was removed by a rotary evaporator (Heidolph Hei-VAP Advantage Rotary Evaporator package) under vacuum, the pellet being lyophilized and stored in a dark glass tube at −20°C until further analysis.
In order to obtain summer savory total TE Soxhlet extraction method was employed. Aerial parts (50 g), crushed and homogenized, were placed in the Soxhlet apparatus (Solvent Extractor™, SER 148 Series; Velp Scientifica, Usmate, Italy) and 400 ml of 70% EtOH were used to sequentially extraction for 48 h. The final extract was filtered through filter paper, the solvent was removed by a rotary evaporator (Heidolph Hei-VAP Advantage Rotary Evaporator package) under vacuum, the pellet being lyophilized and stored in a dark glass tube at −20°C until further analysis.