Standard rat chow

Manufactured by Inotiv

Sourced in United States

Standard rat chow is a complete and balanced feed designed to meet the nutritional requirements of laboratory rats. It provides the necessary macronutrients, vitamins, and minerals to support the general health and well-being of rats in a research or laboratory setting.

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Lab products found in correlation

Sprague dawley rats, by Inotiv (1 mentions) Individually ventilated cages, by Tecniplast (1 mentions) Wistar rat, by Charles River Laboratories (1 mentions) Male sprague dawley rats, by Taconic Biosciences (1 mentions)

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Standard chow (21 citations) Rat chow (9 citations)

8 protocols using standard rat chow

Acclimation and Approval of Sprague Dawley Rats

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Experiments were done in male Sprague Dawley rats (225–250 g) procured from Envigo (Indianapolis, IN). Upon arrival, the rats were permitted to acclimate with free access to water and standard rat chow (Envigo) for 4–5 days while housed under controlled light:dark cycle (lights on 06:00–18:00) and temperature (21–23°C). All husbandry, procedures, and protocols were approved by Wayne State University’s Institutional Animal Care and Use Committee (#19–08-1225) in compliance with ARRIVE guidelines and the National Research Council’s Guide for the Care and Use of Laboratory Animals.

Das S., Komnenov D., Newhouse L., Rishi A.K, & Rossi N.F. (2022). Paraventricular Nucleus V1a Receptor Knockdown Blunts Neurocardiovascular Responses to Acute Stress in Male Rats after Chronic Mild Unpredictable Stress. Physiology & behavior, 253, 113867.

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Sprague-Dawley Rat Feeding Study

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Ten male Sprague-Dawley rats (n = 10) were sourced from the University of Otago’s Hercus Taieri Resource Unit (Dunedin, New Zealand) and housed in 38 × 30 × 35 cm clear plexiglass, individually ventilated cages (Tecniplast, Italy). At the beginning of experiments, rats were approximately 7 months of age with an average body weight of 439 g (±5.8 g). At the end of experiments, rats were approximately 10 months of age with an average body weight of 436 g (±5.1 g). Animals were paired in cages but kept separate by a clear, perforated barrier so auditory and olfactory interaction could happen, but no direct physical contact could occur. All animals were kept on a 12 h reverse dark-light cycle, with all experimental procedures being conducted during the animal’s dark phase. The rats were kept on a restricted diet of standard rat chow (Teklad diet, Envigo, United States) to limit their body weight and promote interest in food reward; all rats were maintained at ≥85% of their free-feeding body weight. Water was available ad libtum. All procedures were approved by the Animal Ethics Committee at the University of Otago, protocol 91/17.

Silva C., Porter B.S, & Hillman K.L. (2021). Stimulation in the Rat Anterior Insula and Anterior Cingulate During an Effortful Weightlifting Task. Frontiers in Neuroscience, 15, 643384.

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Wistar Rat Acclimatization Protocol

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All procedures were carried out in accordance with The Animals (Scientific Procedures) Act, 1986. The ARRIVE guidelines were followed for reporting the use of animals in scientific experiments.⁵² Local ethical approval was granted by The University of Manchester Animal Welfare Ethical and Review Board. Female Wistar rats (250–275 g) were purchased from Charles River, and maintained at the University of Manchester. All animals were allowed to acclimatize for at least 1 week upon arrival before use. Animals were group‐housed in individually ventilated cages under standard conditions, with a regular 12:12 h light/dark cycle. Access to food (standard rat chow, Envigo) and water was provided ad libitum.

Smith K.L., Swiderska A., Lock M.C., Graham L., Iswari W., Choudhary T., Thomas D., Kowash H.M., Desforges M., Cottrell E.C., Trafford A.W., Giussani D.A, & Galli G.L. (2022). Chronic developmental hypoxia alters mitochondrial oxidative capacity and reactive oxygen species production in the fetal rat heart in a sex‐dependent manner. Journal of Pineal Research, 73(3), e12821.

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Wistar Rat Exercise Training Protocol

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Three-month-old male Wistar rats (initial weight: 334.6 ± 3.9 g) were obtained from the Animal Quarters of the Autonoma University of Madrid (Registration number EX-021U; ES-280790000097). This study was carried out in accordance with the recommendations of the European Union guidelines on the ethical care of experimental animals (DG XI of the European Commission, Directive 2010/63/EU, of September 22, 2010, Annex IV). The protocol was approved by the Ethics Committee of the Autonoma University of Madrid (CEI-38-839; RD 1201/2005). Rats were housed at a constant room temperature, humidity, and light cycle (12:12-h light-dark) and had free access to tap water and standard rat chow (#2014, Harlan Teklad, MN, United States) ad libitum. Animals were weighed every 2 weeks. Rats were divided into two experimental groups: (1) control group (C; n = 10) and (2) rats trained for 8 weeks (ET; n = 10). Rats were fasting overnight for 12 h before euthanasia. After being sacrificed the muscle soleus and the interscapular BAT pads were removed, weighed, and washed in saline solution, dried and immediately frozen and stored at -80°C.

de las Heras N., Klett-Mingo M., Ballesteros S., Martín-Fernández B., Escribano Ó., Blanco-Rivero J., Balfagón G., Hribal M.L., Benito M., Lahera V, & Gómez-Hernández A. (2018). Chronic Exercise Improves Mitochondrial Function and Insulin Sensitivity in Brown Adipose Tissue. Frontiers in Physiology, 9, 1122.

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Long-Evans Rat Habituation and Exposure

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Adult male Long-Evans rats (n=32) and intact adult female Long-Evans rats (n=12) (Harlan, Indianapolis, IN, USA; 100–120 days of age upon arrival) were obtained and allowed to habituate in individual housing in shoebox cages for three weeks before exposure to VBS. Water and food were provided ad libitum in a temperature/humidity-controlled room on a 12/12-h light/dark cycle. Rats were fed standard rat chow (Harlan Teklad, Indianapolis, IN, USA) and provided tap water for the duration of the experiment. All procedures were carried out in accordance with the guidelines of the National Institutes of Health (NIH) and were approved by the Institutional Animal Care and Use Committee of the University of Cincinnati.

Makinson R., Lundgren K.H., Seroogy K.B, & Herman J.P. (2015). Chronic social subordination stress modulates glutamic acid decarboxylase (GAD) 67 mRNA expression in central stress circuits. Physiology & behavior, 146, 7-15.

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Rat Developmental Neuroscience Protocol

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Female Sprague-Dawley rats (N = 135) were obtained from a breeding colony at the University of Nebraska at Omaha. Day of birth was designated P0 and litters were culled to a maximum of 12 rats per litter. Pups were weaned at P25 and maintained ad libitum on standard rat chow (Teklad) and water. Each surgical condition was comprised of animals from at least two different litters. All procedures were carried out under the approval of the University of Nebraska at Omaha Institutional Animal Care and Use Committee and in full accordance with NIH guidelines.

Omelian J.M., Berry M.J., Gomez A.M., Apa K.L, & Sollars S.I. (2015). Developmental Time Course of Peripheral Cross-modal Sensory Interaction of the Trigeminal and Gustatory Systems. Developmental neurobiology, 76(6), 626-641.

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Sprague Dawley Rat Estrous Cycle Study

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Male and female Sprague Dawley rats (Charles Rivers Laboratory) were used throughout. Animals were age-matched upon arrival into the facility and all testing occurred between 75-100 days of age. During the early part of the light phase animals were weighed, and vaginal cytology was monitored in the females as previously described (Santollo et al., 2017 (link)). Cycle stage determinations corresponded to the previous 12-h dark phase and the subsequent 12-h light phase (Becker et al., 2005 (link)). Only rats with 4-day estrous cycles were included in the experiments. Animals were pair housed in standard shoebox cages until three days before the commencement of behavioral tests. All animals had ab libitum access to standard rat chow (Teklad 2018) and water and animals in Experiments 2-4 had access to 1.5% saline, unless otherwise noted. The colony room was maintained on a 12:12 h light dark cycle (lights off at 1900 h). All experimental protocols were approved by the Animal Care and Use Committee at the University of Kentucky, and the handling and care of individual animals was in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.

Santollo J., Myers K.E., Rainer I.L, & Edwards A.A. (2019). Gonadal hormones in female rats protect against dehydration-induced memory impairments in the novel object recognition paradigm. Hormones and behavior, 114, 104547.

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Husbandry of Sprague-Dawley Rats

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Male Sprague–Dawley (SD) rats (Taconic, Petersburgh, New York) were housed in a temperature- and humidity-controlled facility with a 12 hour light: 12-hour dark, light cycle. Standard rat chow (#7012, Harlan Teklad, Madison, Wisconsin) and tap water were provided ad libitum. All procedures utilizing experimental animals were conducted in accordance with the Guide for the Care and Use of Laboratory Animals, and experimental procedures were reviewed and approved by the Institutional Animal Care and Use Committee at Merck Research Laboratories, Rahway, New Jersey.

Zhou X., Huang C.H., Lao J., Pocai A., Forrest G., Price O., Roy S., Kelley D.E., Sullivan K.A, & Forrest M.J. (2015). Acute hemodynamic and renal effects of glucagon-like peptide 1 analog and dipeptidyl peptidase-4 inhibitor in rats. Cardiovascular Diabetology, 14, 29.

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