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Knockout serum 20

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Knockout Serum 20 is a cell culture supplement that is commonly used to inhibit the growth and proliferation of specific cell lines. It is designed to selectively remove undifferentiated cells from a mixed cell population, allowing for the enrichment of differentiated cell types.

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (2 mentions) Streptomycin, by Thermo Fisher Scientific (2 mentions) Penicillin, by Thermo Fisher Scientific (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Dispase, by Thermo Fisher Scientific (2 mentions) Dorsomorphin, by Merck Group (2 mentions) Sb431542, by Bio-Techne (2 mentions) β mercaptoethanol, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Knockout serum (54 citations) DMEM/F12 + 20% KnockOut-Serum Replacement (4 citations)

2 protocols using knockout serum 20

1

Generation of Human Cortical Spheroids from iPSCs

Cited 1 time
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Human cortical spheroids were generated from iPSC as previously described31 (link). Briefly, iPSC lines derived from two healthy control individuals were grown on inactivated mouse embryonic fibroblast feeders in the following medium: DMEM/F12, Knockout Serum 20%, 1 mM non-essential amino acids (1:100), GlutaMax (1:200), β-mercaptoethanol (0.1 mM), penicillin and streptomycin (1:100) (Life Technologies). Cultures were regularly tested and maintained mycoplasma free. Colonies of iPSCs were detached intact with dispase (0.35 mg/ml, Invitrogen) and transferred into low-attachment plates in iPSC medium supplemented with dorsomorphin (5 μM, Sigma) and SB-431542 (10 μM, Tocris), and the medium was changed daily. On day six of in vitro differentiation, neural spheroids were transferred to NPC medium (Neurobasal A, B27 without vitamin A, GlutaMax (1:100), penicillin and streptomycin; Life Technologies), which was supplemented with EGF (20 ng/ml) and FGF2 (20 ng/ml) until day 24, and then supplemented with BDNF (20 ng/ml) and NT3 (20 ng/ml) from day 25 to 42. From day 43 onwards, cortical spheroids were maintained in NPC medium, which was changed every 4 days.
Deverman B.E., Pravdo P.L., Simpson B.P., Kumar S.R., Chan K.Y., Banerjee A., Wu W.L., Yang B., Huber N., Pasca S.P, & Gradinaru V. (2016). Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology, 34(2), 204-209.
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2

Generation of Human Cortical Spheroids from iPSCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Human cortical spheroids were generated from iPSC as previously described31 (link). Briefly, iPSC lines derived from two healthy control individuals were grown on inactivated mouse embryonic fibroblast feeders in the following medium: DMEM/F12, Knockout Serum 20%, 1 mM non-essential amino acids (1:100), GlutaMax (1:200), β-mercaptoethanol (0.1 mM), penicillin and streptomycin (1:100) (Life Technologies). Cultures were regularly tested and maintained mycoplasma free. Colonies of iPSCs were detached intact with dispase (0.35 mg/ml, Invitrogen) and transferred into low-attachment plates in iPSC medium supplemented with dorsomorphin (5 μM, Sigma) and SB-431542 (10 μM, Tocris), and the medium was changed daily. On day six of in vitro differentiation, neural spheroids were transferred to NPC medium (Neurobasal A, B27 without vitamin A, GlutaMax (1:100), penicillin and streptomycin; Life Technologies), which was supplemented with EGF (20 ng/ml) and FGF2 (20 ng/ml) until day 24, and then supplemented with BDNF (20 ng/ml) and NT3 (20 ng/ml) from day 25 to 42. From day 43 onwards, cortical spheroids were maintained in NPC medium, which was changed every 4 days.
Deverman B.E., Pravdo P.L., Simpson B.P., Kumar S.R., Chan K.Y., Banerjee A., Wu W.L., Yang B., Huber N., Pasca S.P, & Gradinaru V. (2016). Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain. Nature biotechnology, 34(2), 204-209.
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