V30m mutated ttr

Recombinant human wild-type and V30M (mutated) TTR were purchased from Wako (Osaka, Japan). To generate aggregated wild-type or mutated TTR, each TTR was incubated as described previously^{17 (link), 28 (link)}. Briefly, each TTR (pH 4.0) was incubated at 37 °C for 24 h. To confirm production of aggregated TTR, thioflavin T (ThT)-based fluorimetric assays were performed. Aggregated wild-type or mutated TTR was diluted in 50 mM glycine/NaOH buffer (pH 9.5) containing 5 μM ThT, and ThT fluorescence intensity measured using a Hitachi F-2700 fluorescence spectrophotometer (Hitachi, Tokyo, Japan) (excitation wavelength, 442 nm; emission wavelength, 489 nm). Generation of aggregated TTR from wild-type and mutated TTR was confirmed (Supplementary Figure S4).

Recombinant human wild-type and V30M (mutated) TTR were purchased from Wako (Osaka, Japan). To generate aggregated wild-type or mutated TTR, each TTR was incubated at pH 4.0 for 24 h [33 (link)].