Anti prsk

Protein was detected using primary antibodies detecting YBX1, CDK6, TCTP, GAPDH, H3, and pYBX1 (ab138654 and ab138654 detect endogenous levels of YB1 only when phosphorylated at S102), pAKT, pERK, pRSK, AKT, ERK, RSK, PDGFRB, PCK2, and BAD. Anti-pAKT, anti-pERK, anti-pRSK, anti-AKT, anti-ERK, and anti-RSK were purchased from Cell Signaling Technology (CST) and all the other antibodies were purchased from Abcam. Western blotting was performed as in previous studies (Li et al, 2016 (link)). Briefly, protein samples collected above were separated on SDS–PAGE gels via electrophoresis, transferred to PVDF membranes (Millipore), blocked, and probed using appropriate primary antibodies. Samples were then probed with appropriate secondary antibodies and protein bands were detected using the ECL reagent (GE Healthcare). GAPDH was used as a loading control for total and cytoplasmic protein, whereas H3 was used for nuclear protein. ImageJ was used to quantify protein band densitometry.