Anti gfap rabbit pab

Manufactured by Wuhan Servicebio Technology

Anti-GFAP rabbit pAb is a primary antibody product used for the detection and quantification of glial fibrillary acidic protein (GFAP) in various samples and applications. GFAP is an intermediate filament protein that is considered a specific marker for astrocytes, a type of glial cell in the central nervous system.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using anti gfap rabbit pab

Immunohistochemical Analysis of Hippocampal Neuroinflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brains were rapidly removed, frozen at −80 °C, and cut into 2-mm coronal slices in a rat brain matrix. After 10% formalin fixation, hippocampal tissue samples were processed into paraffin-embedded blocks and 5-μm-thick sections were cut for immunostaining. Hippocampal CA1 region sections were deparaffinized and treated with EDTA antigen repair buffer (pH 8.0). Antibodies used were anti-ionized calcium binding adaptor molecule 1 (IBA-1; anti-Iba1 rabbit pAb, Servicebio, HRP anti-rabbit IgG (HRP-conjugated goat anti-rabbit IgG, Servicebio), anti-glial fibrillary acidic protein (anti-GFAP; anti-GFAP rabbit pAb, Servicebio), and Cy3 anti-rabbit (Cy3-conjugated goat anti-rabbit IgG, Servicebio). Finally, the sections were sealed with the antifluorescence solution (G1401-5ML, Servicebio), and the immunofluorescence images were observed and recorded with fluorescence microscope (NIKON ECLIPSE C1, NIKON DS-U3).

Li P., Huang W., Yan Y.N., Cheng W., Liu S., Huang Y., Chen W., Chen Y.P., Gao Y., Lu W., Xu Y, & Meng X. (2021). Acupuncture Can Play an Antidepressant Role by Regulating the Intestinal Microbes and Neurotransmitters in a Rat Model of Depression. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 27, e929027-1-e929027-12.

+ Open protocol

+ Expand

Multi-Modal Analysis of Neural Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

The PZT was obtained from SCH Technology Co. Ltd. The 443 nm μ-LEDs were obtained from Shenzhen Rico Photoelectric Technology Co., Ltd. The red μ-LEDs were obtained from Shenzhen Super high-brightness Electronics Co., Ltd. The following reagents and antibodies were used in the study: Anti- IBA 1 Mouse mAb (Servicebio, GB12105; diluted: 1:100), Cy3 conjugated Goat Anti-mouse IgG (H + L) (Servicebio, GB21301; diluted: 1:100), Anti- GFAP Rabbit pAb (Servicebio, GB11096; diluted: 1:100), fluorescein isothiocyanate (FITC)–conjugated goat anti-rabbit IgG secondary antibody (Servicebio, GB22303; diluted: 1:100), TMR (Red) Tunel Cell Apoptosis Detection Kit (Servicebio, G1502-50T; Recombinant TdT enzyme: TMR-5-dUTP Labeling Mix: Equilibration Buffer (1: 5: 50)), Hematoxylin (Servicebio, G1004), Eosin Y (Biobomei, YE2080), 4′,6-diamidino-2-phenylindole (Servicebio, G1012), Toluidine blue (Servicebio, G1032).

Zhong T., Yi H., Gou J., Li J., Liu M., Gao X., Chen S., Guan H., Liang S., He Q., Lin R., Long Z., Wang Y., Shi C., Zhan Y., Zhang Y., Xing L., Zhong J, & Xue X. (2024). A wireless battery-free eye modulation patch for high myopia therapy. Nature Communications, 15, 1766.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!