Anti phospho nurr77 s351

Manufactured by Cell Signaling Technology

Anti-Phospho-Nurr77 (S351) is a primary antibody that specifically recognizes the phosphorylated form of the Nurr1 (NR4A2) transcription factor at serine 351. This antibody can be used to detect and quantify the phosphorylation of Nurr1 in various biological samples.

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Lab products found in correlation

Anti actin, by Merck Group (2 mentions) Anti phospho creb s133, by Cell Signaling Technology (2 mentions) Anti o glcnac, by Abcam (2 mentions) Anti rsk1 rsk2 rsk3, by Cell Signaling Technology (2 mentions) Anti phospho erk1 t202 y204 erk2 t185 y187, by R&D Systems (2 mentions) Anti erk1 erk2, by R&D Systems (2 mentions) Anti phospho p90rsk s380, by Cell Signaling Technology (2 mentions) Anti nurr77, by Novus Biologicals (2 mentions) Anti creb, by Cell Signaling Technology (2 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Dual luciferase assay protocol, by Promega (1 mentions)

2 protocols using anti phospho nurr77 s351

Western Blot Antibody Protocol

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Antibodies for Western blotting were used as follows: anti-O-GlcNAc (No. ab2739-Abcam): 1:1000; Anti-actin (No. A2066, Sigma Aldrich), 1:1000; Anti-Phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (No. AF1018, R&D Systems), 1:500; Anti-ERK1/ERK2 (No. AF1576, R&D Systems), 1:500; Anti Phospho-p90RSK (S380) (No. 9341S, Cell Signaling), 1:500; Anti-Rsk1/rsk2/rsk3 (No. 9355T, Cell Signaling), 1:500; Anti-Phospho-Nurr77 (S351) (No. 5095S, Cell Signaling), 1:500; Anti-Nurr77 (No. NB100-56745, Novus Biologicals), 1:500; Anti-Phospho-CREB (S133) (No. 9198S, Cell Signaling), 1:500; Anti-CREB (No. 9104S, Cell Signaling), 1:500.

Massman L.J., Pereckas M., Zwagerman N.T, & Olivier-Van Stichelen S. (2021). O-GlcNAcylation Is Essential for Rapid Pomc Expression and Cell Proliferation in Corticotropic Tumor Cells. Endocrinology, 162(12), bqab178.

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Luciferase Assay for Pomc Promoter

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Antibodies for western blotting are used as follows: anti-O-GlcNAc (#ab2739-Abcam): 1:1000; Anti-actin (#A2066, Sigma Aldrich), 1:1000;
Anti-Phospho-ERK1(T202/Y204)/ERK2(T185/Y187) (#AF1018, R&D systems), 1:500; Anti-ERK1/ERK2 (#AF1576,R&D systems), 1:500; Anti Phospho-p90RSK (S380) (#9341S, Cell Signaling), 1:500; Anti-Rsk1/rsk2/rsk3 (#9355T, Cell Signaling), 1:500;
Anti-Phospho-Nurr77 (S351) (#5095S, Cell Signaling), 1:500; Anti-Nurr77 (#NB100-56745, Novus Biologicals), 1:500; Anti-Phospho-CREB (S133) (#9198S, Cell Signaling), 1:500; Anti-CREB (#9104S, Cell Signaling), 1:500.
AtT-20 cells were concurrently transfected using Lipofectamine 3000 (Thermofisher) with two plasmids -one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (-646bp to +65bp) (#17553, Addgene). Forty-eight hours after transfection, luciferase activity was measured using the dual-luciferase assay protocol (#E1910, Promega). Transfection was performed 48h before measurement of the promoter activity.

Massman L., Pereckas M., Zwagerman N.T., & Stichelen S.O. (2021). O-GlcNAcylation is essential for rapid Pomc expression and cell proliferation in corticotropic tumor cells.

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