Mouse CD8+ T cells were isolated from tumor tissue in Ebag9KO and control mice using mouse CD8α-positive selection kit (STEMCELL Technologies). The CD8+ T cells were intravenously injected into C57BL/6 mice (2 × 106 cells per mouse), after 2 days, the mice were inoculated with MB-49 cells (5 × 105 cells per mouse) that were suspended in 50 μl of PBS with 50 μl of Matrigel into the flank. Tumor volume was measured once or twice a week. The results are shown as mean values±s.d. Statistical analysis was carried out using Student's t-test.
Mouse cd8α positive selection kit
Manufactured by STEMCELL
Sourced in France, Canada
The Mouse CD8α-positive selection kit is a laboratory tool used for the isolation and enrichment of CD8α-positive cells from mouse samples. It provides a simple and effective method to separate these specific cell types for further analysis or research purposes.
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3 protocols using mouse cd8α positive selection kit
1
Adoptive Transfer of CD8+ T Cells in Tumor Model
2
Isolation of Mouse CD8+ T Cells
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Spleens and bone marrows (femurs and tibias) from donor mice were harvested and homogenized in RPMI 1640 medium containing 10% FBS and 1% Penicillin/Streptomycin ( = complete medium). Red blood cells were lysed using sterile filtered RBC lysis buffer (eBioscience, San Diego, USA) and cells were washed, resuspended in phosphate buffered saline (PBS) containing 3% FBS, and filtered through a 70 µM nylon membrane. For CD8 T-cell depletion, the “Mouse CD8α positive selection kit” (Stem Cell, Grenoble, France) was used according to the manufacturer's EASYSEP depletion protocol. Finally, cells were suspended in 200 µl PBS for i.v. injection.
3
Isolation and Expansion of Mouse CD8+ T Cells
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Mouse CD8+ T cells were isolated from tumor tissue using a mouse CD8α-positive selection kit (STEMCELL Technologies, Vancouver, BC, Canada), according to the manufacturer's protocol. Isolated CD8+ T cells were activated and expanded using a T-cell activation/expansion kit (Miltenyi Biotec, Bergisch Gladbach, Germany).
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