Twenty-four hours after the surgery, muscularis externa from the small intestine was isolated and enzymatically digested in MEMα medium (Lonza) containing 100 µg/ml of Penicillin, 100 µg/ml of Streptomycin, 50 µM β-mercaptoethanol, 5% FCS, 5 mg/ml protease type I (Sigma-Aldrich), 20 mg/ml collagenase type II (Sigma-Aldrich) and 5 U/ml DNase I for 15 min at 37°C. Cell suspensions were pre-incubated with an anti-FcR antibody (clone 24G2; BD Biosciences) and then stained with the following antibodies: CD45-APC-eFluor780 (30.F11, eBioscience), CD11b-PE-Cy7 (M1/70, BD Biosciences), CD64-Alexa Fluor647 (X54-5/7.1, BD Biosciences), Ly6G-PercPCy5.5 (IA8, BD Biosciences) and Ly6C-PE (AL-21, BD Biosciences). Stained samples were analyzed on a BD FACSCanto™ flow cytometer (BD Biosciences), and data were processed using FlowJo software (version 10.0.6, Tree Star). Cell numbers were calculated from flow cytometry frequencies using hemocytometer counts of trypan blue–excluding cells.
Ly6c pe al 21
Manufactured by BD
Ly6C-PE (AL-21) is a fluorochrome-conjugated antibody that binds to the Ly6C antigen. Ly6C is a cell surface marker expressed on various cell types, including monocytes, neutrophils, and subsets of T cells. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of Ly6C-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Dnase 1, by Merck Group (1 mentions)
Facscanto flow cytometer, by BD (1 mentions)
Collagenase type 2, by Merck Group (1 mentions)
Cd45apcefluor780 30 f11, by Thermo Fisher Scientific (1 mentions)
Memα medium, by Lonza (1 mentions)
Protease type 1, by Merck Group (1 mentions)
Anti fcr antibody clone 24g2, by BD (1 mentions)
Mdsc isolation kit, by Miltenyi Biotec (1 mentions)
Sb203580 hy 10256, by MedChemExpress (1 mentions)
2 protocols using ly6c pe al 21
1
Isolation and Analysis of Intestinal Immune Cells
2
Phenotypic Analysis of MDSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant murine CXCL10 (250‐16) was purchased from PeproTech. Directly conjugated anti‐mouse mAbs CD11b‐FITC (M1/70), CD45‐PE/Cy7 (30‐F11), Ly6G‐APC/Cy7 (1A8), and Ly6C‐PE (AL‐21) were from BD Pharmingen. Annexin V‐APC apoptosis analysis kit (AO2001‐11P‐G) was obtained from Sungene. The MDSC isolation kit was from Miltenyi Biotec. Anti‐Erk (Ser473, #4696), anti‐p‐Erk (#5726), anti‐JNK (#9252), anti‐p‐JNK (Thr183/Tyr185, #9255), anti‐p38 (#8690), and anti‐p‐p38 (Thr180/Tyr182, #4511) Abs were purchased from Cell Signaling Technology. SB203580 (HY‐10256) were purchased from Med Chem Express.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!