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3 protocols using gallic acid monohydrate acs reagent

1

Antibacterial Properties Evaluation of HPMC

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Hydroxypropyl methylcellulose E15 (HPMC E15; AnyCoat-C AN15®) was purchased from Lotte Fine Chemical (Ulsan, Korea). Folin–Ciocalteau phenol reagent, gallic acid monohydrate ACS reagent (≥98.0%) and tryptic soy broth were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Sodium bicarbonate (NaHCO3) was purchased from Merck (Darmstadt, Germany). Sodium nitrate (NaNO3) and aluminum chloride (AlCl3) were purchased from Merck (Darmstadt, Germany). Sodium hydroxide (NaOH) was purchased from Ajax Finechem (Victoria, Australia). A brain heart infusion (BHI) broth powder was purchased from HiMedia (Mumbai, India). Clindamycin solution; Clinda-M, RPC® (equivalent to 1% clindamycin base) was purchased from RPC International Co., Ltd. (Bangkok, Thailand).
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2

Antioxidant Potential of Stingless Bee Cerumen

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The cerumen of Tetragonula laeviceps was purchased from the meliponiculturists in Ramphan village, Tha Mai District, Chanthaburi 22170, Thailand, and shipped to the Singapore Institute of Technology, Singapore. Gum Arabic Grade 1 (Hashab Acacia Senegal) was acquired from Ingredion Pte Ltd., Singapore, and Maltodextrin DE 10–20 was purchased from Suntop Enterprise Pte Ltd., Singapore. Folin–Ciocalteu’s phenol reagent, 1,1-Diphenyl-2-picrylhydrazyl Free Radical (DPPH), gallic acid monohydrate ACS reagent, sodium carbonate anhydrous, and analytical grade ethanol (99.9%) were obtained from Sigma-Aldrich, Singapore.
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3

Quantifying Antioxidant Potential in Samples

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The chemical reagents used were: hydrochloric acid solution 32 wt. % in H2O, FCC (Sigma-Aldrich, Merck, Darmstadt, Germany); sodium hydroxide pellets EMPROVE® ESSENTIAL (Merck, Darmstadt, Germany); gallic acid monohydrate ACS reagent ≥98.0% (Sigma-Aldrich, Merck, Darmstadt, Germany); sodium bicarbonate ACS reagent ≥99.7% (Merck, Darmstadt, Germany); methanol ACS EMSURE® (Merck, Darmstadt, Germany); Folin and Ciocalteu's Phenol reagent (MP Biomedicals, Valiant Co. Ltd., China); 2,2-Diphenyl-1-picrylhydrazyl (free radical) 95% (Alfa Aesar, Thermo Fisher Scientific, Kandel, Germany); absolute ethanol ≥99.8%; AnalaR NORMAPUR® ACS (VWR, West Chester, Pennsylvania, United States); formic acid 98% for ACS analysis (PanReac AppliChem, ITW Reagents, Chicago, USA); acetonitrile Optima LC/MS grade (Fisher Chemical, Pittsburgh, PA, USA); and ultrapure water (MilliQ Advantage A10 water purification system, Millipore, Billerica, MA, USA).
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