C631 Human HAP1 parental control cell line and two different HAP1 MTH1 KO cell lines (HZGHC000753c002, 8 bp deletion exon 2; HZGHC000753c003, 10 bp deletion exon 2) were obtained from Horizon Discovery Limited. Cells were maintained in Iscove’s Modified Dulbecco’s Medium with 10% FBS and 1% Penicillin/Streptomycin/Fungizone as directed by the manufacturer.
C631 human hap1 parental control cell line
Manufactured by Horizon Discovery
The C631 Human HAP1 parental control cell line is a genetically unmodified human cell line developed for use as a control in cellular assays. This cell line retains the genetic characteristics of the parental HAP1 cell line and serves as a reference to compare against genetically engineered variants.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Cos 7, by American Type Culture Collection (1 mentions)
Helam, by American Type Culture Collection (1 mentions)
2 protocols using c631 human hap1 parental control cell line
1
HAP1 MTH1 KO Cell Lines
2
Cell Lines and Genetic Manipulations
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HeLa, HeLaM, and HEK293T and COS7 cells were obtained from the American Type Culture Collection. Control. VAP-A/B double knock-out56 (link) and parental HeLa cells were a gift from Prof Pietro De Camilli’s laboratory (Yale University School of Medicine, New Haven, CT, USA). Cells were cultured in DMEM with 4.5 g/l glucose (Lonza) containing 10% heat-inactivated FBS (Gibco) and 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco) during experimental procedures. Human INPP5A 4 bp deletion KO cell line and a C631 human HAP1 parental control cell line was purchased from Horizon Discovery (HZGHC006619c008). Cells were routinely tested for Mycoplasma contamination and all tests were negative.
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