Hp taq dna polymerase

E. coli Top10 was purchased from Invitrogen Biotechnology Co. (Grand Island, NY, USA). E. coli fabD mutant (ΔfabD) was obtained from the Coli Genetic Stock Center from the Yale University (New Haven, CO, USA). E. coli ∆fadD strain was provided by Dr. Pamela Silver, Harvard Medical School (Boston, MA, USA) [21 (link)]. E. coli SHuffle strain was acquired from New England BioLabs (Ipswich, MA, USA) (Table S1). The pCDFDuet-1 vector with the ORF-B of the PUFA synthase from Thraustochytrium sp. 26185 was obtained from our previous research [14 (link)]. Expression vectors, pBAD and pET28a, were purchased from Invitrogen Co. (Carlsbad, CA, USA) (Table S2). DNA purification kit was acquired from Bio Basic Inc. (York, ON, Canada). Q5 polymerase, restriction enzymes and dNTP were purchased from New England Biolabs (NEB) (Ipswich, MA, USA). HP Taq DNA polymerase was purchased from Bio Basic Inc. T4 ligase was acquired from Thermo Fisher Scientific. EcoRI, HindIII, and BglII restriction enzymes were purchased from NEB. Primers for MAT PCR amplifications and site-directed mutagenesis (Table S3) were synthesized by Sigma-Aldrich (St. Louis, MO, USA).

Agrobacterium and E. coli media were purchased from Bio Basic Inc. (York, Ontario, Canada). Fatty acids and their standards were obtained from Nu-Chek-Prep, Inc. (Elysian, MN, USA). Q5 DNA polymerase, restriction enzymes, T7 Endonuclease and deoxynucleotide triphosphate (dNTP) were purchased from New England Biolabs (Ipswich, MA, USA). HP Taq DNA polymerase was obtained from Bio Basic Inc. (York, Ontario, Canada). Primers were synthesized from Sigma-Aldrich (St. Louis, MO, USA). GC grade solvents were from Fisher Scientific (Ottawa, ON, Canada). All other chemicals were purchased from Sigma-Aldrich Ltd (Oakville, ON, Canada). The intermediate vector and expression vector for CRISPR/Cas9 system were purchased from Addgene (Watertown, MA, USA).