Ion xpress plus fragment library kit reagent kit

We used the methodology previously described by Pisarenko et al. [33 (link)]. The preparation of a genomic library with a 400-bp read length was performed using the Ion Xpress Plus Fragment Library Kit reagent kit (Life Technologies, USA) in accordance with the manufacturer’s protocol. DNA library fragments were separated using a ready-to-use commercial kit 2% E-Gel SizeSelect agarose gel (Invitrogen, USA). The finished library of DNA fragments was purified using Agencourt AMPure XP magnetic particles (Beckman Coulter, USA). Library quality and concentration were determined using the Experion™ Automated Electrophoresis System and Experion DNA 1 K Reagents and Supplies and Experion DNA Chips kits (Bio-Rad, USA). Monoclonal amplification on microspheres was performed using Ion PGM Hi-Q View OT2 Kit reagents (Life Technologies, USA) in accordance with the manufacturer’s protocol. Microsphere enrichment was performed using Dynabeads MyOne Streptavidin C1 magnetic particles (Invitrogen, Life Technologies, USA). The effectiveness of the enrichment process was evaluated using the Ion Sphere Quality Control Kit (Life Technologies, USA). Genome sequencing was performed using an Ion Torrent PGM sequencer and Ion 316 Chips Kit V2 chips (Life Technologies, USA).

The preparation of genomic libraries with a 400 bp read length was performed using the Ion Xpress Plus Fragment Library Kit reagent kit (Life Technologies, USA) in accordance with the manufacturer’s protocol. DNA library fragments were separated using 2% E-Gel SizeSelect agarose gel (Invitrogen, USA). The finished libraries of DNA fragments were purified using Agencourt AMPure XP magnetic particles (Beckman Coulter, USA). Libraries quality and concentration were determined using the Experion™ Automated Electrophoresis System and Experion DNA 1 K Reagents and Supplies and Experion DNA Chips kits (Bio-Rad, USA). Monoclonal amplification on microspheres was performed using Ion PGM Hi-Q View OT2 Kit reagents (Life Technologies, USA) in accordance with the manufacturer’s protocol. Microsphere enrichment was performed using Dynabeads MyOne Streptavidin C1 magnetic particles (Invitrogen, Life Technologies, USA). The effectiveness of the enrichment process was evaluated using the Ion Sphere Quality Control Kit (Life Technologies, USA). Genome sequencing was performed using an Ion Torrent PGM sequencer and Ion 318 Chips Kit V2 chips (Life Technologies, USA).