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2 protocols using l tryptophan

1

Quantitative Analysis of Phenolic Compounds

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Methanol, acetonitrile, external standards, such as gallic acid, chlorogenic acid, β- coumaric acid, with a purity of 99.5%, were purchased from Sigma-Aldrich and Fluka (Warsaw, Poland). Tween 20, L-tryptophan, L-phenylalanine, L-tyrosine were purchased from Carl Roth (Karlsruhe, Germany).
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2

Comprehensive Amino Acid Analysis Protocol

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The HPLC-grade acetonitrile, acetic acid, l-aspartic acid, l-glutamic acid, dl-serine, l-histidine, glysine, l-threonine, l-arginine, l-alanine, l-tyrosine, l-valine, l-methionine, l-isoleucine, l-leucine, l-phenylalanine, l-ornithine monohydrochloride, l-lysine monohydrochloride, 4-aminobutyric acid (as internal standard), and DEEMM were obtained from Merck (Darmstadt, Germany). High-purity water was supplied by the PURELAB Option-Q system from Elga Veolia (High Wycombe, UK); sodium acetate was purchased from Carlo Erba Reagents (Val-de-Reuil, France); and ltryptophan was purchased from Carl Roth (Karlsruhe, Germany).
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