Nexera x2 hplc uhplc system

A prominence HPLC instrument (Shimadzu, Kyoto, Japan) was used for chromatographic purification. A Nexera X2 HPLC/UHPLC system (Shimadzu, Japan) was employed for the quantitative analysis. An amaZon speed-ion trap mass spectrometer (Bruker, Billerica, MA, USA) and an AVANCE-I 600 NMR (Bruker) were used for chemical identification. A Yamanaka-type soil hardness tester from Fujiwara Seisakusho, Ltd. (Tokyo, Japan) and soil moisture sensor kit SM150T from Delta-T Devices (Cambridge, UK) were used for the in-situ soil analysis.

The EYELA multi-shaker MMS-type and centrifugal evaporators were obtained from Tokyo Rika Kikai (Tokyo, Japan) and Sakuma Seisakusho Co., Ltd. (Tokyo, Japan). A SpectraMax iD5 microplate reader (Molecular Devices, San Jose, CA, USA) was used for the in vitro assays. A Nexera X2 HPLC/UHPLC system (Shimadzu, Kyoto, Japan) was used for quantitative chromatographic analysis. A Bruker amaZon Speed Ion-Trap Mass Spectrometer (Billerica, MA, USA) was used for chemical identification. For the in situ soil analysis, a soil moisture sensor kit SM150T (Delta-T Devices, Cambridge, UK) and a Yamanaka-type soil hardness tester (Fujiwara Seisakusho, Tokyo, Japan) were used. Soil macronutrients were analyzed using an EW-THA1J soil analyzer (Air Water Biodesign, Osaka, Japan).