Accu check performa meter

Sham and HF rats treated with vehicle or vildagliptin were fasted for 8 h. The blood glucose level was determined from their tail vein using the ACCU-CHECK® Performa meter (Roche Diagnostics GmbH, Mannheim, Germany).

Fasting glucose levels were measured using the ACCU-CHECK^® Performa meter (Roche Diagnostics GmbH, Mannheim, Germany). The serum urea was measured by colorimetric enzymatic hydrolysis by the urease system using a Labtest kit (Labtest, Minas Gerais, Brazil). Serum and urinary creatinine concentrations were measured using a kinetic method (Labtest) and a ThermoPlate Analyzer Plus (ThermoPlate, São Paulo, Brazil). The urinary protein excretion was determined using a Sensiprot kit (Labtest). The urinary albumin concentration was determined using an ELISA kit specific for rat urine albumin (Nephrat kit; Exocell, Philadelphia, PA, USA). The experiments were carried out in accordance with the manufacturer’s instructions.