C2687

Manufactured by Merck Group

Sourced in United Kingdom, United States

The C2687 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use, but its core function is not specified in the information provided. A more detailed and unbiased description is not available.

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Lab products found in correlation

Mab1874, by R&D Systems (1 mentions) Ab199376, by Abcam (1 mentions) Alexa fluor 488 goat anti mouse igg, by Jackson ImmunoResearch (1 mentions) Fab357p, by R&D Systems (1 mentions) Mouse igg1 pe, by R&D Systems (1 mentions) Triton x 100, by Merck Group (1 mentions) Facscalibur, by BD (1 mentions) Donkey anti goat igg alexa fluor 488, by Jackson ImmunoResearch (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Ab89901, by Abcam (1 mentions) Ab14106, by Abcam (1 mentions) Ab14041, by Abcam (1 mentions) Nbp2 33006, by Novus Biologicals (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions)

2 protocols using c2687

Immunofluorescence Staining of Cardiac Cells

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Differentiated cells were fixed with 4% paraformaldehyde (PFA) and permeabilized with 0.4% Triton™ X-100 (No. T8787; Sigma). After blocking in 5% donkey or goat serum, cells were stained with primary antibodies against ISL1 (diluted 1:1,000; sc23590; Santa Cruz, Dallas, TX), cTnT (0.5 μg/mL; MAB1874; R&D, Minneapolis, MN), WT1 (diluted 1:1,000; ab89901; Abcam, Cambridge, UK), CNN1 (diluted 1:10,000; C2687; Sigma), TAGLN (diluted 1:1,000; ab14106; Abcam), POSTN (diluted 1:1,000; ab14041; Abcam), or KDR-PE (10 μL/10⁶ cells; FAB357P; R&D).
Alexa Fluor^® 488 donkey anti-Goat IgG (705-545-147; Jackson ImmunoResearch Laboratories, Inc., West Grove, PA), Alexa Fluor 488 goat anti-rabbit IgG (111-545-003; Jackson), Alexa Fluor 488 goat anti-mouse IgG (115-545-003; Jackson), and PE goat anti-rabbit IgG (GR200G-09C; Sungene Biotech, Tianjin, China) were used as secondary antibodies. Goat IgG (sc3887; Santa Cruz), Rabbit IgG (ab199376; Abcam), mouse IgG1-PE (IC002P; R&D), and mouse IgG1 (M5284; Sigma) were used as isotype controls. Samples were assessed using a FACSCalibur (Becton Dickinson, Franklin Lakes, NJ) and data were analyzed using FlowJo (Treestar).

Zhao J., Cao H., Tian L., Huo W., Zhai K., Wang P., Ji G, & Ma Y. (2017). Efficient Differentiation of TBX18+/WT1+ Epicardial-Like Cells from Human Pluripotent Stem Cells Using Small Molecular Compounds. Stem Cells and Development, 26(7), 528-540.

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Characterizing Smooth Muscle Cell Markers

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For flow cytometry analysis, SMCs were labeled with primary antibodies for anti-α-SMA (1:200, NBP2-33006, Novus biologicals, Centennial, CO, USA), anti-calponin (1:150, C2687, Sigma Aldrich, Buchs, Switzerland), anti-myostatin (1:50, H00002660-M07, Novus biologicals, Centennial, CO, USA) or anti-Isotype IgG mouse (1:150, sc-2877, Santa Cruz, Heidelberg, Germany). The cells were incubated with the secondary antibodies anti-mouse FITC (1:200, BD 55988, BD Biosciences, Allschwil, Switzerland) or anti-rabbit FITC (1:200, FP-SA5000 Alexa 488, Brunschwig, Switzerland). Analysis was conducted using an LSR Fortessa^TM cell analyzer (BD Bioscience, Allschwil, Switzerland) and results were analyzed with FlowJo software 7.2.4 (Tree Star Inc., Ashland, OR, USA).

Salemi S., Schori L.J., Gerwinn T., Horst M, & Eberli D. (2023). Myostatin Overexpression and Smad Pathway in Detrusor Derived from Pediatric Patients with End-Stage Lower Urinary Tract Dysfunction. International Journal of Molecular Sciences, 24(5), 4462.

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