Starting from the cell-free supernatants of the vaginal swabs, the concentration of IL-6 (pg/ml) and IL-8 (pg/ml) was determined by means of commercial ELISA assays (Simple Plex Human IL-6 and IL-8 Cartridges) run on Ella automated immunoassay system (R&D systems, Minneapolis, United States), following the manufacturer’s instructions (Brys et al., 2020 (link)).
Ella automated immunoassay system
Manufactured by R&D Systems
Sourced in United States
The Ella automated immunoassay system is a laboratory instrument designed for the automated processing of immunoassays. It is capable of performing various types of immunoassays, such as ELISA, in a high-throughput manner. The system automates the essential steps of an immunoassay, including sample handling, reagent addition, incubation, and detection, to provide consistent and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Simple plex human il 6, by R&D Systems (1 mentions)
Il 8 cartridges, by R&D Systems (1 mentions)
Tnfr1, by R&D Systems (1 mentions)
Human il 10 duoset elisa, by R&D Systems (1 mentions)
Human il 10 quantikine elisa kit, by R&D Systems (1 mentions)
Gm csf, by R&D Systems (1 mentions)
Duoset elisa, by R&D Systems (1 mentions)
Elisa maxtm deluxe set human il 6, by BioLegend (1 mentions)
5 protocols using ella automated immunoassay system
1
Quantifying Vaginal Cytokine Levels
2
Quantifying Cytokine Levels in Lung Perfusion
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Perfusate, BAL, and lung IL-10 levels were determined with a human IL-10 Quantikine ELISA kit (R&D Systems, Minneapolis, MN, USA), and in vitro cell media IL-10 levels were determined with a human IL-10 DuoSet ELISA (R&D Systems). Perfusate cytokine panel analysis was performed with an Ella automated immunoassay system (R&D Systems) using 16×4 multianalyte cartridges for IL-1β, IL-6, IL-8, endothelin-1, GM-CSF, TNFR1, and TREM-1 (R&D Systems).
3
Cytokine Secretion Evaluation in Cell Cocultures
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Supernatants of co-cultures of cells with hPBMCs were analysed by ELISA assays to evaluate the secretion of interleukin 6, interleukin-2, IFN-γ, and granzyme B. Briefly, after the treatments in the absence or presence of immunomodulatory mAbs, supernatants were centrifuged and treated for quantification of human IL-6 (ELISA MAXTM Deluxe Set Human IL-6, BioLegend, San Diego, CA, USA), human IL-2, IFN-γ, and granzyme B (DuoSet ELISA, R&D Systems, Minneapolis, MN, USA), according to the producer’s recommendations. ELISA assays to measure TNF-α cytokine level were performed by using Ella Automated Immunoassay system (R&D Systems, Minneapolis, MN, USA), following the producer’s recommendations. Concentration values were reported as the mean of at least three determinations.
4
Measuring Cytokines and CRP in Vaccination
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TNF and IL-6 were measured in participant plasma pre-vaccination using the Ella Automated Immunoassay System and Simple Plex 2nd generation Human TNF and IL-6 cartridges (R&D Systems, MN, USA). Samples were randomized prior to plating, and a single biological replicate was included on each cartridge to assess inter-assay variability. The inter-assay CV was 4% for TNF, and 14% for IL-6 and both measures are presented as pg/ml. CRP was measured by immunoturbidimetric method at the Health Sciences North clinical laboratory; given the lower limit of detection for this assay (5 mg/L, as opposed to high-sensitivity CRP), participants were categorized as low or undetectable (< 5 mg/L), medium (5–10 mg/L) and high (10 or more mg/L), the latter being the upper limit of normal [70 (link)]. CMV serostatus was determined in serum using a CMV IgG ELISA kit (Genesis Diagnostics Inc., Cambridgeshire, UK) according to the manufacturer’s instructions.
5
Inflammatory Biomarker Profiling in Rodents
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