Thunder imager live cell

Manufactured by Leica

The Thunder Imager Live cell is a high-performance microscopy system designed for live-cell imaging. It provides real-time, high-resolution visualization of dynamic cellular processes. The system's core function is to enable researchers to capture and analyze live-cell data with exceptional clarity and precision.

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Lab products found in correlation

Cryostat, by Leica (1 mentions) Arginase 1, by Thermo Fisher Scientific (1 mentions) Gr 1 clone rb6 8c5, by BioLegend (1 mentions) Z fix, by Thermo Fisher Scientific (1 mentions) Dithiothreitol (dtt), by Merck Group (1 mentions)

Close spelling variants of this product

Thunder Imager (61 citations) Thunder Imager 3D live cell microscope (8 citations) THUNDER Imager 3D Live Cell and 3D Cell Culture (3 citations) THUNDER Imager 3D Live Cell (2 citations) THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay (2 citations)

2 protocols using thunder imager live cell

Quantifying Tumor Immune Infiltrates

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Subcutaneous tumors were fixed with Z-fix (Fisher Scientific) and frozen in OCT (Fisher Scientific). Samples were cut into 8 µm sections using a cryostat (Leica) and analyzed by immunofluorescence using antibodies against Arginase 1 (Life Technologies) and Gr-1 (clone RB6-8C5, BioLegend). Sections were imaged using Leica Thunder Imager Live cell and 3D Assay microscope.

Cardot-Ruffino V., Bollenrucher N., Delius L., Wang S.J., Brais L.K., Remland J., Keheler C.E., Sullivan K.M., Abrams T.A., Biller L.H., Enzinger P.C., McCleary N.J., Patel A.K., Rubinson D.A., Schlechter B., Slater S., Yurgelun M.B., Cleary J.M., Perez K., Dougan M., Ng K., Wolpin B.M., Singh H, & Dougan S.K. (2023). G-CSF rescue of FOLFIRINOX-induced neutropenia leads to systemic immune suppression in mice and humans. Journal for Immunotherapy of Cancer, 11(6), e006589.

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Immunofluorescence Analysis of Dispersed Chromosomes

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ZP-free oocytes were fixed in a solution containing 1% paraformaldehyde, 0.15% Triton X-100, and 3 mM DTT (Sigma-Aldrich) on glass slides for 30 min. After fixation, slides were air-dried. To perform immunofluorescence analysis of dispersed chromosomes, slides were blocked with 1% BSA and then incubated for 1 hr with primary antibodies. After rinsing three times, slides were incubated with secondary antibodies for 45 min, followed by DAPI staining. Slides were then mounted and examined with a Leica THUNDER Imager Live Cell with a K5 camera driven by the Leica Application Suite Software. Image processing was performed by ImageJ software. See antibodies information in Key resources table.

Huang L., Li W., Dai X., Zhao S., Xu B., Wang F., Jin R.T., Luo L., Wu L., Jiang X., Cheng Y., Zou J., Xu C., Tong X., Fan H.Y., Zhao H, & Bao J. (2023). Biallelic variants in MAD2L1BP (p31comet) cause female infertility characterized by oocyte maturation arrest. eLife, 12, e85649.

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