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Anti pakt 66444 1 ig

Manufactured by Proteintech
Sourced in China

Anti-pAKT (66444-1-Ig) is a primary antibody for the detection of phosphorylated AKT (also known as protein kinase B) in various applications such as western blotting, immunohistochemistry, and immunofluorescence.

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3 protocols using anti pakt 66444 1 ig

1

TRPM2 Overexpression and Signaling

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pcDNA3.1-TRPM2-S-Flag was synthesized by Youbio Co., Ltd. The following antibodies were used in this study: anti-TRPM2-L rabbit polyclonal antibody (ab11168; Abcam); anti-TRPM2-S & -L rabbit polyclonal antibody (bs-2888R; Bioss); anti-Flag (K200001M; Solarvbio); anti-PTEN (60300-1-Ig; Proteintech); anti-pAKT (66444-1-Ig; Proteintech); anti-pGSK3β (#9327S; Cell signaling technology); anti-β-Caternin (#8480S; Cell signaling technology); anti-NF-Kβ (#3033S; Cell signaling technology); anti-pERK (#4370S; Cell signaling technology); anti-Bcl-2 (#3498S; Cell signaling technology); anti-cleaved caspase3 (#9664S; Cell signaling technology); anti-GAPDH mouse monoclonal antibody (60004-1-Ig; Proteintech).
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2

Immunoblotting analysis of phosphorylated proteins

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Primary antibodies against P-JNK (4668T), P-ERK (4370), P-P38 (4511T), and β-actin (4970) were from Cell Signalling Technology (Danvers, USA). Anti-P-Akt (66444-1-Ig) was obtained from Proteintech. Commercial tobacco Daqianmen was purchased from Shanghai Jieqiang Tobacco, Sugar and Wine (Group) Co., Ltd.
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3

Protein Expression Analysis in Prostate Cancer

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PCa tissue samples and DU145 and 22RV1 cells were lysed by RIPA
(radio-immunoprecipitation assay) buffer (P0013B; Beyotime, Shanghai, China).
The supernatants were resolved in SDS-PAGE and transferred onto polyvinylidene
fluoride (PVDF) membranes (IPVH00010; Millipore, Billerica, MA, USA), incubated
with anti-METTL3 (DF12020; Affinity, Changzhou, China), anti-PI3K (AF6241,
Affinity), anti-AKT (60203-2-Ig; Proteintech, Wuhan, China), anti-p-AKT
(66444-1-Ig; Proteintech), and anti-glyceraldehyde-3-phosphate dehydrogenase
(GAPDH; AB-P-R 001; Hangzhou, China) overnight at 4°C. Protein bands were
scanned by enhanced chemiluminescence (ECL).
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