Anti pakt 66444 1 ig
Anti-pAKT (66444-1-Ig) is a primary antibody for the detection of phosphorylated AKT (also known as protein kinase B) in various applications such as western blotting, immunohistochemistry, and immunofluorescence.
Lab products found in correlation
3 protocols using anti pakt 66444 1 ig
TRPM2 Overexpression and Signaling
Immunoblotting analysis of phosphorylated proteins
Protein Expression Analysis in Prostate Cancer
(radio-immunoprecipitation assay) buffer (P0013B; Beyotime, Shanghai, China).
The supernatants were resolved in SDS-PAGE and transferred onto polyvinylidene
fluoride (PVDF) membranes (IPVH00010; Millipore, Billerica, MA, USA), incubated
with anti-METTL3 (DF12020; Affinity, Changzhou, China), anti-PI3K (AF6241,
Affinity), anti-AKT (60203-2-Ig; Proteintech, Wuhan, China), anti-p-AKT
(66444-1-Ig; Proteintech), and anti-glyceraldehyde-3-phosphate dehydrogenase
(GAPDH; AB-P-R 001; Hangzhou, China) overnight at 4°C. Protein bands were
scanned by enhanced chemiluminescence (ECL).
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