pcDNA3.1-TRPM2-S-Flag was synthesized by Youbio Co., Ltd. The following antibodies were used in this study: anti-TRPM2-L rabbit polyclonal antibody (ab11168; Abcam); anti-TRPM2-S & -L rabbit polyclonal antibody (bs-2888R; Bioss); anti-Flag (K200001M; Solarvbio); anti-PTEN (60300-1-Ig; Proteintech); anti-pAKT (66444-1-Ig; Proteintech); anti-pGSK3β (#9327S; Cell signaling technology); anti-β-Caternin (#8480S; Cell signaling technology); anti-NF-Kβ (#3033S; Cell signaling technology); anti-pERK (#4370S; Cell signaling technology); anti-Bcl-2 (#3498S; Cell signaling technology); anti-cleaved caspase3 (#9664S; Cell signaling technology); anti-GAPDH mouse monoclonal antibody (60004-1-Ig; Proteintech).
Anti pakt 66444 1 ig
Manufactured by Proteintech
Sourced in China
Anti-pAKT (66444-1-Ig) is a primary antibody for the detection of phosphorylated AKT (also known as protein kinase B) in various applications such as western blotting, immunohistochemistry, and immunofluorescence.
Automatically generated - may contain errors
Lab products found in correlation
Ab11168, by Abcam (1 mentions)
Anti bcl 2, by Cell Signaling Technology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti p erk, by Cell Signaling Technology (1 mentions)
Anti p gsk 3β, by Cell Signaling Technology (1 mentions)
β actin 4970, by Cell Signaling Technology (1 mentions)
Ipvh00010, by Merck Group (1 mentions)
3 protocols using anti pakt 66444 1 ig
1
TRPM2 Overexpression and Signaling
2
Immunoblotting analysis of phosphorylated proteins
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Primary antibodies against P-JNK (4668T), P-ERK (4370), P-P38 (4511T), and β-actin (4970) were from Cell Signalling Technology (Danvers, USA). Anti-P-Akt (66444-1-Ig) was obtained from Proteintech. Commercial tobacco Daqianmen was purchased from Shanghai Jieqiang Tobacco, Sugar and Wine (Group) Co., Ltd.
3
Protein Expression Analysis in Prostate Cancer
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PCa tissue samples and DU145 and 22RV1 cells were lysed by RIPA
(radio-immunoprecipitation assay) buffer (P0013B; Beyotime, Shanghai, China).
The supernatants were resolved in SDS-PAGE and transferred onto polyvinylidene
fluoride (PVDF) membranes (IPVH00010; Millipore, Billerica, MA, USA), incubated
with anti-METTL3 (DF12020; Affinity, Changzhou, China), anti-PI3K (AF6241,
Affinity), anti-AKT (60203-2-Ig; Proteintech, Wuhan, China), anti-p-AKT
(66444-1-Ig; Proteintech), and anti-glyceraldehyde-3-phosphate dehydrogenase
(GAPDH; AB-P-R 001; Hangzhou, China) overnight at 4°C. Protein bands were
scanned by enhanced chemiluminescence (ECL).
(radio-immunoprecipitation assay) buffer (P0013B; Beyotime, Shanghai, China).
The supernatants were resolved in SDS-PAGE and transferred onto polyvinylidene
fluoride (PVDF) membranes (IPVH00010; Millipore, Billerica, MA, USA), incubated
with anti-METTL3 (DF12020; Affinity, Changzhou, China), anti-PI3K (AF6241,
Affinity), anti-AKT (60203-2-Ig; Proteintech, Wuhan, China), anti-p-AKT
(66444-1-Ig; Proteintech), and anti-glyceraldehyde-3-phosphate dehydrogenase
(GAPDH; AB-P-R 001; Hangzhou, China) overnight at 4°C. Protein bands were
scanned by enhanced chemiluminescence (ECL).
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