250,000 undifferentiated MØP cells were seeded onto a 12-well plate and 6h later stimulated with LPS (1μg/mL) or zymosan (25μg/mL) overnight. The differentiated MØP cells were stimulated 12h before harvesting. MEF cells were seeded at a density of 200,000 cells per well on a 6-well plate. After 24h, cells were transfected as described above. On the next day, mEFs were stimulated with PMA (200nM) for 2h. In the cell-free supernatant IL-6, TNFα and sTNFRII were measured by ELISA according to the manufacturer’s instructions. The TNFRII ELISA kit was from R&D Systems. The ELISA kits for IL-6 and TNFα were from ebioscience.
Elisa kits for il 6 and tnfα
Manufactured by Thermo Fisher Scientific
The ELISA kits for IL-6 and TNFα are laboratory equipment designed to detect and quantify the levels of these specific cytokines in biological samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to provide reliable and reproducible results.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using elisa kits for il 6 and tnfα
1
Cytokine Secretion by Stimulated Macrophages
2
Inflammatory Cytokine Response Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A total of 250,000 undifferentiated MØP were seeded onto a 12-well plate and stimulated 6 h later with LPS (1 μg/ml) or zymosan (25 μg/ml) overnight. The differentiated MØP were stimulated 12 h before harvesting. mEF cells were seeded at a density of 200,000 cells per well on a six-well plate. After 24 h, cells were transfected as described above. On the next day, mEF were stimulated with PMA (200 nM) for 2 h. In the cell-free supernatant IL-6, TNF-α and soluble TNFRII were measured by ELISA, according to the manufacturer’s instructions. The TNFRII ELISA kit was from R&D Systems. The ELISA kits for IL-6 and TNF-α were from eBioscience.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!