Emx1 ires cre tm1 cre krj

All animal procedures conformed to standards set forth by the Public Health Service policy of the NIH, and were approved by the IACUC at Duke University. 59 mice (both sexes; 3-30 months old; singly and group housed (1-4 in a cage) under a regular 12-h light/dark cycle; C57/B6J (Jackson Labs #000664) was the primary background with up to 50% CBA/CaJ (Jackson Labs #000654)) were used in this study. Pvalb-cre (tml(cre)Arbr, Jackson Labs #008069; n=39; PV::Cre), VGAT-ChR2-EYFP (Slc32a1-COP4*H134R/EYFP, Jackson Labs #014548; n=7), Gad2-IRES-cre (Gad2tm2(cre)Zjh, Jackson Labs #010802; n = 4; GAD::Cre), Emx1-IRES-Cre (tm1(cre)Krj, Jackson Labs # 005628; n=2) and Wild-type (n=1) were used for in vivo extracellular electrophysiology (n= 27), and behavior (n=31) experiments (note five mice were used in both behavior and electrophysiology). For calcium imaging experiments, six mice transgenically expressing GCaMP6 were used (Ai93 [tm93.1(tetO-GCaMP6f)Hze; Jackson Labs #024103] were crossed to Emx1-IRES-Cre and CaMK2a-tTA (Jackson Labs #003010; n=1) and Ai162 [tm162.1(tetO-GCaMP6s,CAG-tTA2)Hze; Jackson Labs #031562] were crossed to Slc17a7-IRES2-Cre-D [tm1.1(Cre)Hze; Jackson Labs #023527; n=5).

Experiments were conducted using 18 mice of both sexes older than P60. C57/BL6J was the primary background with up to 50% CBA/CaJ (Jackson Labs #000654)). GCaMP6 was expressed either transgenically (Ai162 [tm162.1(tetO-GCaMP6s,CAG-tTA2)Hze; Jackson Labs #031562] crossed to Slc17a7-IRES2-Cre-D [tm1.1(Cre)Hze; Jackson Labs #023527], n=10) or virally (Emx1-IRES-Cre [tm1(cre)Krj, Jackson Labs # 005628], n=3; Pvalb-IRES-Cre [tm1(cre)Arbr, Jackson Labs #017320], n=5). All animal procedures were approved by Duke University Institutional Animal Care and Use Committee.