Jb1 catheter

A blunt dissection was performed in anesthetized rabbits to gain access to the right or left common femoral artery. A 3-French vascular sheath (Cook Medical) was placed and a 2-French microcatheter (JB1 catheter, Cook Medical) enabled manipulation into the celiac axis followed by a celiac artery angiogram to delineate hepatic blood supply. Using digital subtraction angiography (DSA) and cone-beam CT angiography (C-arm, Allura Clarity FD20 8.2, Philips), a region of hypervascular blush located in the left liver lobe was identified as the tumor. A steerable guide wire (0.014 in. Transcend wire, Boston Scientific) was used to selectively catheterize the tumor-feeding artery, and final positioning of the catheter was confirmed with DSA.

Two interventional radiologists (P.R. and O.P.) performed the embolization according to conventional protocols. Briefly, a 3-French sheath (Cook, Inc., Bloomington, IN) was introduced into the femoral artery. A 2-French catheter (JB1 catheter; Cook, Inc. Bloomington, IN) was then advanced into the aorta and to the celiac axis, where a selective arteriogram was performed. The left hepatic artery was then selectively catheterized with the aid of a steerable guide wire (0.014 in, Transcend™ wire; Boston Scientific Oncology, Natick, MA). From this position, 1mL of DEB/contrast medium of doxorubicin suspension/contrast medium was slowly injected under fluoroscopic guidance until the entire volume was delivered or stasis was observed (average doxorubicin delivered in both DEB-TACE groups = 12.5 mg). Upon completion of the intra-arterial injection, the catheter was removed and the sheath was left in place until the completion of blood draw sampling for the pharmacokinetic study.

A blunt dissection done in previously anesthetized rabbits allowed access to the right or left common femoral artery, as previously described (13 (link)–16 (link)). Briefly, a 3-F vascular groin sheath (Cook Medical, Bloomington, Indiana) was used to advance a 2-F microcatheter (JB1 catheter; Cook) into the celiac axis followed by a celiac arteriogram to delineate the blood supply of the liver. The target lesion and feeding vessels were identified using either digital subtraction angiography or dual-phase cone-beam CT (C-arm; Allura Clarity FD20, XtraVision Release 8.2; Philips, Best, The Netherlands). After initial mapping, a 0.014-inch guide wire (Transend; Boston Scientific, Marlborough, Massachusetts) was used to selectively catheterize the tumor-feeding artery. Embolization endpoints were defined as complete blood flow stasis. Upon completion of the procedure, the microcatheter was removed, the common femoral artery was ligated, and the cut-down was closed.