Polycarbonate track etch membrane

DOPG as sodium salt in chloroform (25 mg.mL⁻¹, 319 μL) was placed in a round-bottomed flask and 10 mL chloroform was added to increase the total volume (the lipid film formed upon evaporation is more spread on the surface of the round-bottomed flask if the initial volume is large). Chloroform was slowly evaporated under vacuum at 40 °C using a rotary evaporator and then placed 4 h under high-vacuum. The lipids were hydrated using PBS buffer. The turbid suspension of multilamellar vesicles was subsequently extruded 7 times through a 200 nm polycarbonate track-etch membrane and 10 times through a 100 nm polycarbonate track-etch membrane (Whatman) using a 10 mL Thermobarrel extruder (Lipex Biomembranes). The LUV solution was used from the day after their preparation and within one week. They were stored at 4 °C.