Ketamine and xylazine were purchased from Phoenix Pharmaceuticals Inc., (St Joseph, MI). Buprenorphine was purchased from Rickitt & Colman (Richmond, VA). Dura-Pen was from Vedco Inc. (Overland Park, KS). 17β-estradiol sulfate, Propylpyrazole triol (PPT) and 2,3-bis(4-hydroxyphenyl)-proprionitrile (DPN) were purchased from Sigma Aldrich (St. Louis, MO). G1 was purchased from Tocris Biosciences (Ellisville, MO). The ER agonists were dissolved in DMSO for stock solution and the working concentration was a 1:50 dilution in Sterile saline. Sterile saline was purchased from B. Braun Medical (Irvine, CA).
Propylpyrazole triol ppt
Manufactured by Merck Group
Propylpyrazole triol (PPT) is a chemical compound used in laboratory settings. It serves as a core functional component for various applications. The specific details and intended uses of PPT are not available in this unbiased and factual description.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Opti mem, by Thermo Fisher Scientific (2 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions)
Xylazine, by Phoenix Pharmaceuticals (1 mentions)
17β estradiol sulfate, by Merck Group (1 mentions)
Ketamine, by Phoenix Pharmaceuticals (1 mentions)
Sterile saline, by B. Braun (1 mentions)
Durapen, by Vedco (1 mentions)
17β estradiol, by Merck Group (1 mentions)
Uo126, by Selleck Chemicals (1 mentions)
M mlv rt, by Promega (1 mentions)
Ly290004, by Selleck Chemicals (1 mentions)
Enhanced chemi luminescence (ecl), by Thermo Fisher Scientific (1 mentions)
Sp600125, by Merck Group (1 mentions)
Mg132, by Selleck Chemicals (1 mentions)
Gotaq qpcr master mix, by Promega (1 mentions)
Rnasin, by Promega (1 mentions)
3 protocols using propylpyrazole triol ppt
1
Estrogen Receptor Agonist Administration
2
Estrogen Receptor Agonists and Mitochondrial Function
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Two weeks after the GDX, the animals were treated once intraperitoneally with E2 (17β-estradiol, Sigma Aldrich; 2,6 µg per 25 -g mouse), or with a specific ERα agonist [propyl pyrazole triol (PPT), Sigma Aldrich; 5,2 µg per 25 -g mouse], or with a specific ERβ agonist (BAY-121-4257, 2,6 µg per 25 -g mouse). These concentrations were chosen to achieve the E2 level within a physiological range, as we had done previously (21 (link), 22 (link)).
At the end of the experiment, all mice were sacrificed. Hearts and musculus solei were isolated for mitochondria respiration measurements, and the uteri have been removed to assess the response to OVX.
At the end of the experiment, all mice were sacrificed. Hearts and musculus solei were isolated for mitochondria respiration measurements, and the uteri have been removed to assess the response to OVX.
3
Syndecan-1 Regulation in Breast Cancer
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DMEM, Ham-F12 medium and antibiotics were purchased from Dominique DUTSCHER SAS (Brumath, France). Goat polyclonal (AF2780) anti-human Syndecan-1 antibody was obtained from R&D Systems. ESR1-antibody (F10) was from Santa Cruz Biotechnology and anti-Actin (Ab-1) from Millipore. Fetal bovine serum (FBS), Opti-MEM, Lipofectamine RNAiMax, ECL were purchased from Thermo-Fisher Scientific. M-MLV-RT, RNasin, GoTaq qPCR Master-Mix were from Promega. Positively charged nylon membrane (Amersham Hybon-N+, GE Healthcare, Life Sciences). E2, propyl-pyrazole-triol (PPT), diarylpropionitrile (DPN), G1, G15 and SP600,125, fluoroshield were from Sigma. Ly290,004, UO126, BAY 11-7085 (Bay) and MG-132 were purchase from Selleckchem.
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