Kallikrein

The following coagulation and coagulation-related proteases were purchased from Enzyme Research Labs (USA): kallikrein and thrombin [also called factor (F) IIa, FXa, FXIa, and FXIIa]. The following chromogenic substrates were purchased from Diapharma (USA): for thrombin, S-2238; for FXa, S-2765; for FXIa and APC, S-2366; and for kallikrein and FXIIa, S-2302. Custom synthetic double-stranded DNA fragments (gBlocks) were bought from Integrated DNA Technologies (Canada). Restriction endonucleases and glutathione agarose were purchased from Thermo Fisher Scientific (Canada). Nickel chelate affinity resin Ni-NTA agarose was bought from Qiagen (Canada). PreScission Protease [a glutathione sulfotransferase (GST)–human rhinovirus (HRV) 3C protease fusion protein] was purchased from GE Healthcare (Canada). Normal human pooled plasma (NHPP) was produced in-house. FXI-deficient plasma was purchased from Affinity Biologicals (Canada). STA PTTA reagent, STA Neoplastine CI Plus reagent, and Owren-Koller buffer were bought from Diagnostica Stago (Canada).

Purified human coagulation proteases FXIa, thrombin, FXa, FXIIa, kallikrein, and APC were from Enzyme Research Labs (South Bend, IN, USA). Chromogenic substrates were purchased from Diapharma (West Chester, OH, USA): for FXIa and APC, S-2366; for kallikrein and FXIIa, S-2302; for thrombin, S-2238; and for FXa, S-2765.