Log In Sign up
The largest database of trusted experimental protocols

Ki 67 12202

Manufactured by Cell Signaling Technology
Visit Supplier

Ki-67 (12202) is an antibody product offered by Cell Signaling Technology. It is used for the detection of the Ki-67 protein, which is a cellular marker for proliferation. The antibody can be utilized in various applications, such as immunohistochemistry and immunofluorescence, to study cell proliferation.

Automatically generated - may contain errors

Lab products found in correlation

Bcl 2 sc 7382, by Santa Cruz Biotechnology (1 mentions) β actin a2228, by Merck Group (1 mentions) 3 3 diaminobenzidine peroxidase substrate kit, by Vector Laboratories (1 mentions) Decloaking chamber, by Biocare Medical (1 mentions) Dako protein block, by Agilent Technologies (1 mentions) Anti cleaved caspase 3 5a1e, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-Ki-67 (Catalog #12202 S, Ki-67

3 protocols using ki 67 12202

1

Diethylnitrosamine-induced signaling pathway analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Diethylnitrosamine (DEN), (catalogue number: N0258; MilliporeSigma, Burlington, MA) was dissolved in saline and stored at 4°C until used. Antibodies specific to pSTAT3Tyr705 (9145), STAT3 (12640), pERK1/2Thr202/Tyr204 (4370), ERK1/2 (4695), pGSK-3α/βSer21/9 (9331), GSK-3α/β (9315), p-c-JunSer73 (3270), c-Jun (9165), pIGF-IRTyr1135/1136 (3024), IGF-IR (9750), Ki-67 (12202) (Cell Signaling, Cambridge, MA), BCL-2 (sc-7382), BCL-xL/xS (sc-1041) (Santa Cruz Biotechnology, Delaware, CA), and β-Actin (A2228), (MilliporeSigma) were used.
Haque A., Sahu V., Lombardo J.L., Xiao L., George B., Wolff R.A., Morris J.S., Rashid A., Kopchick J.J., Kaseb A.O, & Amin H.M. (2022). Disruption of Growth Hormone Receptor Signaling Abrogates Hepatocellular Carcinoma Development. Journal of Hepatocellular Carcinoma, 9, 823-837.
+ Open protocol
+ Expand
2

Immunohistochemical Analysis of Mouse Colon

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sections from large intestines were collected from 6-week-old mice. Paraffin sections (5 μm) were deparaffinized in xylene and rehydrated in an ethanol gradient. Antigen retrieval was performed in Rodent Decloaker using a Decloaking Chamber (Biocare Medical, Walnut Creek, CA). Endogenous peroxidase activity was quenched by incubation in 3% H2O2 for 10 min, and slides were blocked with Dako Protein Block (DakoCytomation Inc., Mississauga, Ontario, Canada) for 30 min at room temperature. The slides were incubated with primary antibody for 1 h at room temperature. The following polyclonal antibodies were used: anti-cleaved caspase-3 (5A1E, 1:800) and Ki-67 (12202, 1:400) (Cell Signaling Technology, Beverly, MA). After washing, slides were incubated with prediluted biotinylated goat anti-rabbit IgGs (Biocare Medical) for 30 min and then treated with 3,3′-diaminobenzidine peroxidase substrate kit (Vector Laboratories, SK-4100). Samples were mounted with aqueous mounting medium (Dako, S3025), and images were taken on a Nikon microscope using NIS-Elements software.
Soares F., Tattoli I., Rahman M.A., Robertson S.J., Belcheva A., Liu D., Streutker C., Winer S., Winer D.A., Martin A., Philpott D.J., Arnoult D, & Girardin S.E. (2014). The Mitochondrial Protein NLRX1 Controls the Balance between Extrinsic and Intrinsic Apoptosis. The Journal of Biological Chemistry, 289(28), 19317-19330.
+ Open protocol
+ Expand
3

Adipose Tissue Histology and Immunostaining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Adipose tissues were fixed in 4% PFA for 24 h at 4°C. After dehydration, the tissues were embedded into paraffin and cut into 5 µm thick slices, deparaffinized and rehydrated using standard methods. Sections were stained with hematoxylin & eosin. For immunohistochemical staining, antigen retrieval was performed by submerging in 0.01 M sodium citrate (pH 6.0) and heated to boil for 20 min in a microwave oven. BAT was subjected to immunofluorescence staining and antibodies against UCP1 was purchased from Santa Cruz Biotech (sc-28766), and Ki67 (12202) was purchased from Cell signaling technology.
Yang Q., Liang X., Sun X., Zhang L., Fu X., Rogers C.J., Berim A., Zhang S., Wang S., Wang B., Foretz M., Viollet B., Gang D.R., Rodgers B.D., Zhu M, & Du M. (2016). AMPK/α-ketoglutarate axis dynamically mediates DNA demethylation in the Prdm16 promoter and brown adipogenesis. Cell metabolism, 24(4), 542-554.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!